摘要
目的:快速准确测定黄连-吴茱萸药对中生物碱的血药浓度。方法:采用高效液相色谱-质谱联用法对黄连-吴茱萸药对中盐酸小檗碱、盐酸巴马汀、盐酸药根碱和吴茱萸碱的血药浓度进行测定,以甲醇-水为流动相梯度洗脱,Hypersil GOLD C18柱(50 mm×2.1 mm,3μm)为固定相,流速为0.2 ml/min,柱温为35℃。质谱采用高温电喷雾离子源,以选择反应监测模式进行定量分析,血浆样品用乙腈直接沉淀处理。结果:盐酸小檗碱在1.243～30.578 ng/ml,盐酸巴马汀在1.240～30.541 ng/ml,盐酸药根碱在1.248～30.647 ng/ml,吴茱萸碱在28.432～553.314 ng/ml范围内均呈良好线性关系。日内、日间RSD均小于15%(n=5)。结论:本方法操作简单、灵敏度高、分析时间短,适用于黄连-吴茱萸药对生物碱血药浓度监测及药代动力学研究。
Objective： To determine the content of Alkaloid for Coptis-Evodia couplet medicines in plasma by LC-MS/MS. Methods： A high performance liquid chromatography electrospray mass spectrometry method for rapid determination of Berberine hydrochloride, Pal- matine hydrochloride,Jatrorrhizine hydrochloride and Evodiamine for Coptis-Evodia couplet medicines in plasma has been established. The analysis was carriedon a Hypersil GOLD C18 （50 mm × 2.1 mm, 3 μm） column. The column temperature was 35℃, with linear gradient elution using water and methanol, and the flow rate was 0. 2 ml/min. The quantitation was done by selected reaction recording （SRM） mode of heater eleetrospray ionization （HESI）, with phenytoin sa internal standard. Plasma was deposited by aeetonitrile direct- ly. Results： The linear range were 1. 243 -30. 578 ng/ml for Berberine hydroehloride, 1. 240 ~ 30. 541 ng/ml for Palmatine hydroehlo- ride, 1. 248 ~ 30. 647ng/ml for Jatrorrhizine hydrochloride and 28. 432 ~ 553. 314 ng/ml for Evodiamine ; both intra-and inter-day RSD were less than 15% （ n = 5 ）. Conclusion ： The method is accurate, sensitive, simple, and suitable for study of therapeutic drug monito- ring and pharmaeokinetie.
出处
《江西中医学院学报》
2012年第1期31-33,共3页
Journal of Jiangxi College of Traditional Chinese Medicine
基金
国家自然科学基金(30873369)
广东省自然科学基金(8151009501000001)
关键词
液质联用
盐酸小檗碱
盐酸巴马汀
盐酸药根碱
吴茱萸碱
血药浓度
LC-MS/MS
Berberine hydrochloride
Palmatine hydrochloride
Jatrorrhizine hydrochloride
Evodiamine
Plasma concen-tration
