摘要
确立了玉米芯经稀酸预处理后加水蒸煮提取木聚糖 ,然后再加酶水解提取液的生产低聚木糖的工艺路线 .玉米芯在质量分数为 0 .1%的H2 SO4 溶液中于 6 0℃下浸泡 12h后 ,滤去浸泡液并水洗至 pH 6左右 ,然后采用液固比 10∶1,150℃ ,30min的蒸煮条件进行蒸煮 .结果表明 ,可溶性木聚糖的提取得率达 17% (按玉米芯计 ) ,提取液的RS/TS小于 33% .提取液和渣一起用木聚糖酶进行水解 ,可获得阿拉伯糖 /葡萄糖 /木糖 /木二糖 /木三糖之比为 7.7∶6 .8∶11.5∶54.1∶19.8的高纯度低聚木糖产品 .低聚木糖的质量分数大于 70 % (对总糖 ) ,且产品的可溶性总糖得率达2 6 .4 % (对玉米芯 ) .
The process for production of xylooligosaccharides from corncob was conceived and tested. Corncob was pretreated by 0.1% H 2SO 4 at 60 ℃ for 12 hours, followed by filtration and washing with tap water to about pH 6, then steaming at 150 ℃ for 0.5 h (100 g corncob in 1 L water). The yield of xylan was about 17% of corncob. The extract with the residue was hydrolyzed by specific xylanases. The cumulative yield of xylooligosaccharides after enzymatic hydrolysis was 26.4% of corncob and the ratio of arabinose/ glucose/xylose/xylobiose/xylotriose was 7.7∶6.8∶11.5∶54.1∶19.8.
关键词
玉米芯
木聚糖提取
酶法水解
低聚木糖
corncob
xylan extraction
hydrolysis by xylanases
xylooligosaccharides
