摘要
为满足栽培对种苗的需求,以子叶为材料,采用组织培养的方法,对堇菜进行了愈伤组织诱导与分化、不定芽的分化、不定芽生根与试管苗生根继代增殖、试管苗的移栽和定植的研究,建立起堇菜的无性系。结果证明:MS+BA0.4mg/L+2,4-D丁酯2.5mg/L是子叶愈伤组织的诱导培养和继代增殖培养的理想培养基;MS+NAA0.1mg/L+BA0.6mg/L是愈伤组织分化培养和不定芽分化增殖继代培养的理想培养基;1/4MS+ABT2号2mg/L+NAA0.3mg/L是不定芽生根和试管苗生根继代增殖培养的理想培养基;试管苗移栽成活率为91.9%,定植成活率为99.1%。定植成活的试管苗生长旺盛,并保持了堇菜的所有植物学特征。
The aim was to meet the need for cultivation of germchit.By using tissue culture methods,the cotyledons of Viola verecunda were used as material to do the research on callus induction and differentiation,differentiation and rooting of adventitious buds,rooting and transplanting of tube seedlings,finally establish the clone of Viola verecunda.The results showed that MS＋BA0.4mg/L＋2,4-D2.5mg/L was the optimum medium for inducing callus from cotyledons and subculturing of induced callus.The optimum medium for callus and adventitious buds differentiation was MS＋NAAO.lmg/L＋BA0.6mg/L.1/4MS＋ABT 1/2 mg/L＋NAA0.3mg/L was the ideal medium for rooting and subculturing.The transplanting survival rate of tube seedlings was 91.9% and stable planting survival rate was 99.1%.Colonization of plantlets grew vigorously and maintained for all botanical traits of Viola verecunda.
出处
《内蒙古农业科技》
2012年第4期33-35,共3页
Inner Mongolia Agricultural Science and Technology
基金
辽宁省高等教育教学改革资助项目(20090304)
辽宁师范大学教学改革资助项目(LSJG:20090108)
关键词
堇菜
组织培养
无性系
Viola verecunda
Tissue culture
Clone
