摘要
用ELISA、RIA及DNA斑点杂交法检测30例HBsAg阳性母亲及7例HBsAg阴性但血清其他HBV感染指标阳性母亲的新生儿脐血Anti-HBclgM,HBV-DNA等指标,分别发现4例(4/30)及2例(2/7)胎儿HBV感染病例。1年后的随访结果表明,部分胎儿HBV感染病例对出生后的疫苗注射有反应,而部分则无。用DNA原位杂交及PAP法检测HBsAg阳性产妇胎盘组织HBV-DNA、HBcAg,阳性率分册为20/29,19/30。表明胎盘组织受HBV感染,可能导致胎儿HBV感染,但非胎儿感染HBV的唯一途径,其他途径如经生殖细胞传播也可能存在。HBsAg阴性但其他血清HBV感染指标阳性者及血清HBV感染指标全阴产妇的胎盘组织中也可检出HBV-DNA、HBcAg,表明组织HBV感染指标较敏感,以及HBV相关变型病毒感染等因素存在的可能性。
Placental tissues of HBsAg positive and negative mothers were detected for HBV-DNA and HBcAg using DNA in situ hybridization (ENZO) and immunochemistry(PAP, DAKO), while cord blood of the related neonates was tested for HBV markers by ELISA, RIA and DNA dot blot. Four of 30 neonates whose mothers were HBsAg( + ), and 2 of 7 neonates whose mothers were HBsAg( - ) but other serum HBV markers positive, were ditagno-sed as cases of fetal HBV infection (FHBI). Some of these FHBI cases responded to HBV vaccines after birth, but some did not. Twenty of 29 placentae and 19 of 30 placentae from HBsAg( + ) mothers were positive for HBV-DNA and HBcAg respectively. Placental HBV infection may lead to fetal HBV infection. HBV may infect the fetus through the HBV infected placenta. But other ways of transmission may exist, e. g., through the germ line. HBV-DNA and HBcAg were also detected in the placentae of HBgAg( - ) but other HBV markers positive mothers and serum HBV markers free mothers, indicating that:(i) Tissue HBV markers are more sensitive than serum HBV markers.(2) The existence of the infection of HBV defective virus and HBV related variants is possible.
出处
《中山医科大学学报》
CSCD
1990年第2期23-27,82,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
乙肝病毒
宫内感染
胎儿
胎盘组织
HBV Intrauterine transmission Fetal HBV Infection DNA in situ hybridization
