摘要
:【目的】研究胎儿感染乙型肝炎病毒(HBV)与胎盘感染之间的关系及其意义。【方法】用PCR及斑点杂交法检测母血、新生儿静脉血HBVDNA(确定胎儿感染者),用免疫组化方法(LSAB法)检测61例HBV携带者母亲胎盘HBcAg。【结果】①胎儿HBV感染率为2292%(33/144);②61例胎盘HBcAg阳性率为6885%(41/61),胎儿感染组阳性率(8214%)高于对照组(5455%),P<0025;③母血HBeAg/HBVDNA阳性者,胎盘HBcAg阳性率(8205%)高于母血HBeAg/HBVDNA阴性者(4091%),P<0005;④胎盘各种细胞成分中,以与母血直接接触的蜕膜细胞、末梢绒毛合体细胞及EVT(绒毛外滋养细胞)HBcAg阳性率较高(3607%～4918%)。【结论】胎儿感染HBV与胎盘感染有关,胎盘屏障对HBV有一定阻挡作用。
Objective To study the relationship between fetal infection and placental infection of HBV (Hepatitis B Virus). Method HBV DNA was detected in maternal and fetal peripheral blood by PCR and Southern blot Hybridization. HBcAg was detected in 61 cases of HBV carrier mothers' placentas by immunocytochemistry (LSAB method). Result ① Fetal infection rate of HBV is 22.92%(33/144).② HBcAg positive incidence in carrier mothers' placentas was 68.85% (51/61).But the rate in fetal infection group (82 14%) was higher thanthat in control group (54 55%),P<0 025. ③ The placental HBcAg positive rate in mothers with HBeAg/HBV DNA+(82 05%) was higher than that in mothers with HBeAg HBV DNA (40.91%),P<0.005.④ The frequency of HBcAg positive syncytial cells which contact directly with mothers' blood was the highest among all other kinds of placental barrier cells. Conclusion The HBV was capable of infecting fetus by infecting the placental barrier. The placental barrier can considerably prevent the passage of HBV.
出处
《中山医科大学学报》
CSCD
2000年第1期43-46,53,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
孙逸仙纪念医院科研基金
关键词
乙型肝炎病毒
携带者
孕妇
胎盘
HBCAG
hepatitis B core antigens
hepatitis B virus
disease transmission,vertical
placenta/microbiology
