摘要
以乙醇为溶剂提取瑶山梭罗果实,经减压浓缩后,依次用乙酸乙酯和正丁醇进行萃取。用DPPH自由基的清除能力来评价2个部位的抗氧化活性。结果表明,乙酸乙酯部位浓度为280.80μg/mL时自由基清除率大于56%,IC50=210.21μg/mL,正丁醇部位浓度为368.00μg/mL时自由基清除率大于68%,IC50=291.03μg/mL。乙酸乙酯部位的抗氧化活性是正丁醇部位的1.4倍。
The fruit of Reevesia glaucophylla Hsue were extracted using the ethanol as solvent and then extracted with ethyl acetate and n-butanol successively after vacuum concentration. Scavenging activity of DPPH radical was studied for evaluating antioxidant activity of the two parts. The results showed that radical scavenging rate was greater than 56% and IC50 equaled to 210.21 μg/mL when the part of ethyl acetate concentration was 280.80 μg/mL, and radical scavenging rate was greater than 68% and IC50 equaled to 291.03 μg/mL when the part of n-butanol concentration was 368.00μg/mL. Antioxidant activity of ethyl acetate part is 1.4 times better than that of n-butanol part.
出处
《食品科技》
CAS
北大核心
2012年第8期232-234,共3页
Food Science and Technology
基金
北京林大学合作国家项目(物种10-二-6(01))
贵州省教育厅2010年度自然科学研究基金项目(黔教科2010094)
关键词
瑶山梭罗
果实
抗氧化活性
DPPH自由基
清除率
Reevesia glaucophylla Hsue
fruit
antioxidant activity
DPPH radical
scavenging rate
