摘要
在确定产琥珀酸放线杆菌ATCC55618菌株生长的临界pH为4.5之后,对其进行紫外诱变,筛选出耐pH3.5强酸的菌株M1,产量为17.25g/L,较原始菌株提高了12.09%,之后用含0.1%的溴甲酚绿变色平板筛选,对M1进行两轮紫外-亚硝基胍复合诱变,筛选出琥珀酸高产菌株R2,该菌株琥珀酸产量为27.35g/L,较原始菌株产量提高了77.71%。
Determine the borderline pH 4.5 of Actinobacillus succinogenes ATCC 55618, a mutant strain M1 that tolerance low pH 3.5 was obtained by mutagenesis ultraviolet radiation （UV）, whose yield is 17.25 g/L, improved 12.09% than original strain, and then it was irradiated by both UV and nitrosoguanidine （NTG）, screened by plate that contains 0.1% bromine cresol green （BCG）, After two wheels of the compound mutation, a high producing strain R2 was obtained, whose yield of succinic acid is 27.35 g/L, improved 77.71% than the original strain ATCC 55618.
出处
《食品科技》
CAS
北大核心
2012年第8期18-21,共4页
Food Science and Technology
关键词
琥珀酸
耐酸
高产
诱变
succinic acid
tolerance low pH
high producing
mutagenize
