摘要
为了解古尔班通古特沙漠南缘蜱携带无形体的状况,在该地域3个不同生境区域11个地点采集家畜寄生蜱标本,进行分类鉴定;运用套式PCR筛选无形体阳性蜱样本,并用半套式PCR扩增无形体科16SrRNA基因5′端高变区,进行克隆测序;将所获得的DNA序列与GenBank收录的序列作比对,并用Mega 5.0软件构建系统发育树,鉴定蜱携带无形体的种类。结果共采集708只蜱,鉴定为4个属8个种。在分类后的236份蜱样本中,检测出无形体阳性25份,阳性率为10.59%。序列比较发现,在亚洲璃眼蜱、血红扇头蜱、残缘璃眼蜱、长角血蜱中分别存在查菲埃立克体(Ehrlichia chaffeensis)、犬埃立克体(Ehrlichia canis)、边缘无形体(Anaplasma marginale)和绵羊无形体(Anaplasma ovis)的16SrRNA基因片段。研究结果证实在古尔班通古特沙漠南缘家畜寄生蜱中携带无形体,提示该地域可能是无形体病的自然疫源地。
To understand the tick-borne anaplasmataceae in the south edge of Gurbantunggut desert, ticks specimens from 11 sites in three different habitats areas were collected and identified. Nested PCR was carried to detect positive ticks carring anaplasmataceae,and then 5% end variable region of 16S rRNA gene of anaplasmataceae was amplified by semi-nested PCR for cloning and sequencing. The obtained DNA sequences were compared with the GenBank sequence and phylogenetic tree was constructed with the Mega 5.0 software. The 708 ticks collected from 11 sites were identified as 4 genera and 8 species. 25 were detected positive in the 236 tick samples, with a positive rate of 10.59 %. By sequence analyses, Ehrlichia cha ff eensis , Ehrlichia eanis , Ana plasrna marginale and Ana plasrna ovis were positive in Hyalomrna asiaticum ,Rhipicephalus sanguineeus , Hyalornma detritum and Haemaphysalis longi- cornis,respectively. The results showed that there exists anaplasmataceae in ticks parasited in livestock from the south edge of Gurbantunggut desert,suggesting that the region may be one of natural loci of anaplasmosis.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第8期1158-1163,共6页
Chinese Journal of Veterinary Science
基金
国家基础研究"973计划"资助项目(2010CB530200
2010CB530206)
石河子大学高层次人才科研启动基金资助项目(RCZX2009001)
