摘要
诱导广谱有效的中和抗体被认为是预防性疫苗成功的关键,HIV中和抗体检测方法的建立和标准化为HIV疫苗评价和设计提供指导,也为不同HIV疫苗的比较提供了平台。本文从方法的建立、假病毒株的选择、评价标准的制定、评价策略的确定、该方法在HIV其它研究领域的应用等方面,对目前应用最广泛的以假病毒为基础的中和抗体评价方法进行了综述。
It is well-known that induction of effective broad-spectrum neutralizing antibodies is the key for successful prophylactic vaccines.Establishment and standardization of the HIV neutralizing antibody assays will assist the vaccine design and provide a platform to compare the vaccines from different laboratories.In this paper,we reviewed the most acceptable pseudovirus-based neutralization assay in following aspects：establishment of the assay,principles for pseudovirus selection,determination of the evaluation criteria and strategy,and applications in other fields.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第8期1498-1502,共5页
Chinese Journal of Pharmaceutical Analysis
基金
“艾滋病和病毒性肝炎等重大传染病防治”国家科技重大专项:艾滋病疫苗评价技术的研究(2012ZX10004701-001)
