摘要
目的探索小鼠品系、性别和被毛对利用活体成像技术所建立可视化假病毒感染模型的影响。方法将携带Fluc报告基因的马尔堡假病毒(pMARV-Fluc)经腹腔分别感染C57BL/6J小鼠和BALB/c小鼠,利用活体成像技术分析脱毛前后活体化学发光水平的差异。同时,利用埃博拉假病毒(pEBOV-Fluc)经腹腔途径感染不同性别的C57BL/6J小鼠和BALB/c小鼠,并对峰值发光水平和动态变化过程进行比较分析。结果 BALB/c小鼠的白色被毛对发光水平的遮挡比例为32%, C57BL/6J小鼠的黑色被毛对发光水平的遮挡比例达到82%,差异具有统计学意义(P<0.001)。相比雌性动物而言,雄性动物活体发光水平更强且一致性更好(P<0.05)。BALB/c小鼠发光峰值水平显著高于C57BL/6J(P<0.05),并表现出不同的动态变化过程,峰值时间点分别为4 d和6 d。结论小鼠品系、性别和被毛均对假病毒感染模型有影响。
Objective To investigate the effects of strain, gender, and hair coat of mice upon establishing a bioluminescent imaging based pseudovirus mouse model. Methods C57BL/6 J and BALB/c mice were infected with pseudotyped Marburg virus by intraperitoneal(ip) injection. The bioluminescent signal of mice was collected before and after removing hair to show the influence of hair upon the bioluminescent imaging signal. Meanwhile, C57BL/6 J and BALB/c mice of different gender were infected with pseudotyped Ebola virus by ip and the dynamic changing process of bioluminescence over time was observed. Results The black hair coat of C57BL/6 J mice blocked82% of the bioluminescent signal while the white hair coat of BALB/c mice blocked 32%, the blocking rates are statistically different between C57BL/6 J mice and BALB/c mice(P<0.001). The bioluminescent signal of male mice was stronger(P<0.05) and more homogeneous than the females.The dynamic changing process of bioluminescence showed that the peak of signals of BALB/c mice were reached on the fourth day after infection while those of C57BL/6 J mice were observed on the sixth day after infection. Conclusion The strain, gender and hair of mice have significant influence upon the bioluminescent imaging based pseudovirus mouse model.
作者
雷山
刘强
黄维金
王佑春
LEI Shan;LIU Qiang;HUANG Wei-jin;WANG You-chun(Division of HIV/AIDS and Sex-transmitted Virus Vaccines,National Institutes for Food and Drug Control,Key Laboratory of Calibration Methods and Standardization of Biotechnology Products of the Ministry of Health,Beijing 102629,China;National Vaccine and Serum Institute,Beijing 100176,China)
出处
《实验动物与比较医学》
CAS
2019年第6期423-428,共6页
Laboratory Animal and Comparative Medicine
基金
艾滋病和毒性肝炎等重大传染病防治国家科技重大专项(2017ZX10304402)
