摘要
目的了解2010年昆明医科大学第一附属医院检验科临床分离菌对常用抗生素的耐药性.方法采用K-B法和仪器法对昆明医科大学第一附属医院2010年临床分离菌2 644株进行药敏试验,WHONET5.5统计软件对数据进行分析.结果在检出的2 644株菌株中,住院患者占96%,门诊患者占4%.细菌检出率分别为革兰阴性菌72%,革兰阳性菌28%.革兰阳性菌葡萄球菌62%,肠球菌27%,肺炎链球菌2%.革兰阴性菌中肠杆菌科细菌64%,非发酵菌35%.标本中分离的细菌最多见者(前10位)依次为大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌、金黄色葡萄球菌、表皮葡萄球菌、屎肠球菌、嗜麦芽窄食单胞菌、阴沟肠杆菌、粪肠球菌.未发现VRSA菌株.检出VRE菌株1株.MRSA的检出率为61%,MRCNS的检出率为80%.检出肺炎链球菌18株,未发现PNSP菌株.大肠埃希菌、克雷伯菌属产ESBLS菌株的检出率分别为67%和63%.铜绿假单胞菌的泛耐药菌株检出率为12%,鲍曼不动杆菌的泛耐药菌株检出率为14%.结论定期进行耐药性监测有助于了解昆明医科大学第一附属医院细菌耐药性变迁,为临床用药提供依据.
Objective To understand the resistance of bacteria isolated from patients to the commonly used antibiotics in our hospital in 2010. Methods The drug sensitive test of 2644 strains of bacteria isolated clinically was performed by using k-b method and instruments. WHONET5.5 statistical software was used for data analysis. Results In detected 2644 strains isolated, hospitalized patients accounted for 96%, and clinic patients accounted for 4%. The detection rate was 72% for gram-negative bacteria, and 28% for gram-positive bacteria. In Gram-positive bacteria, Staphylococcus aureus accounted for 62%, enterococcus for 27% and streptococcus pneumoniae for 2%. In Gram-negative bacteria, enterobacteria accounted for 64% and non - fermentative bacteria for 35%. The top 10 most frequently isolated bacteria were as following： escherichia coli, klebsiella pnenmoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, staphylococcus aureus, staphylococcus epidermidis, Enterococcus faecium, Stenotrophomonas maltophilia, Enterobacter cloacae and Enterococcus faeealis. VRSA strain was not found, and 1 VRE strain was found. The detection rate was 61% for MRSA and 80% for MRCNS. 18 strains of streptococcus pneumoniae was detected and no PNSP strain was found. The detection rate was 67% for escherichia coli and 63% for klebsiella pneumoniae. The detection rate was 12% for Pseudomonas aeruginosa drug-resistant strains, and 14% for acinetobacter baumannii drug-resistant strains. Conclusion Regular bacteria resistance monitoring help us to understand the changes of bacteria resistance in our hospital, and provide the basis for clinical drug use.
出处
《昆明医学院学报》
2012年第7期58-63,共6页
Journal of Kunming Medical College
基金
云南省基础研究计划基金资助项目(208CD019)
关键词
耐药性检测
抗药性
药敏试验
Resistance test
Drug resistance
Drug sensitive test
