摘要
目的研究人工培养云南虫草菌粉(yunnan cordyceps sinensis,YNCS)的免疫调节作用.方法 ICR小鼠随机分组,每天用不同剂量的YNCS灌胃,用环磷酰胺(CY)诱导免疫功能低下模型,通过观察免疫器官、吞噬细胞功能、淋巴细胞增殖、血清凝集素、T细胞亚群等指标变化来判断YNCS对机体的免疫调节作用.结果 YNCS对小鼠的胸腺指数和脾指数有上调作用;YNCS低剂量对小鼠腹腔巨噬细胞吞噬功能有增强作用;低、中剂量YNCS组ConA、LPS刺激淋巴细胞增殖比模型对照组显著增高(P<0.01);YNCS低、中剂量对小鼠L3T4+T淋巴细胞有上调作用,并使L3T4+淋巴细胞和L3T4+/Lyt2+比值恢复接近正常水平.YNCS低、中、高剂量组对小鼠凝集素有明显提高作用(P<0.01).结论 YNCS可以上调免疫功能抑制小鼠胸腺指数和脾指数,促进T淋巴细胞的增殖,调节小鼠T细胞亚群,增加血清凝集素水平,从而调节小鼠免疫功能.同时,高剂量的YNCS对免疫有一定的抑制作用.
Objective To study the immunoregulation effects of culture Yunnan Cordyceps sinensis （YNCS） in ICR mouse. Methods Different dosages of YNCS were intragastriced to ICR mice daily which were randomly divided into several groups. The low-immunity ICR mouse model was established by eyelophosphamide （CY） , and then we observed the effect of YNCS on the thymus weight index, spleen weight index, the function of M ~phagocytosis, lymphocyte proliferation, T lymphocyte subpopulation and serum agglutinin of the mouse, in order to judge immunoregulation effects of YNCS. Results YNCS increased the thymus weight index and spleen weight index. The low dose of YNCS enhanced the function of M ~ phagocytosis; the low and mid-dose of YNCS obviously increased the proliferation of ConA and LPS stimulated T lymphocytes compared with blank control group （P 〈 0.01） ; the low and mid-dose of YNCS increased the effect of L3T4~ T lymphocyte and promoted L3T4＋/Lyt-2＋ ratio to recover to normal level. YNCS at low, middle and high dose significantly improved the serum level of agglutinin in mice （P〈 0.01 ）. Conclusions YNCS could increase the thymus weight index and spleen weight index. YNCS could promote proliferating of T lymphocyte and regulate the T lymphocyte subpopulation of the mouse, and improve the serum level of agglutinin. High dose of YNCS could inhibit the immune function to some extent.
出处
《昆明医学院学报》
2012年第7期45-48,共4页
Journal of Kunming Medical College
基金
云南省教育厅科研基金资助项目(07C41064)
关键词
云南
冬虫夏草
免疫调节
Yunnan
Cordyceps Sinensison （YNCS）
Immunoregulation
