摘要
[目的]测定核桃壳多糖的含量。[方法]以葡萄糖为对照品,采用苯酚-硫酸法在490 nm测定核桃壳中多糖含量。[结果]最佳显色条件为5%苯酚1.0 ml,浓硫酸6.0 ml,反应温度65℃,反应时间为30 min,平均回收率为101.9%,RSD为1.98%,线性范围10～90μg/ml。[结论]该方法简便、准确、重现性好,适用于核桃壳多糖的含量测定。
[Objective] To determine the content of polysaccharide in walnut shell.[Method] The content of polysaccharide was determined by phenol-sulfuric acid method at 490 nm with the glucose as reference substance.[Result] The optimal color conditions were 1.0 ml 5% phenol,6.0 ml sulfuric acid,65 ℃ reaction temperature and 30 min reaction time,the average recovery was 101.9%,RSD=1.98% and the liner range was 10-90 μg/ml.[Conclusion] The method is simple,accurate and has good reproducibility,and it is reliable to determine the total polysaccharide in the walnut shell.
出处
《安徽农业科学》
CAS
2012年第25期12654-12655,共2页
Journal of Anhui Agricultural Sciences
基金
云南省科技厅应用基础研究项目(2010ZC137)
大理学院大学生科研基金项目(DXSKYX201003)
关键词
核桃壳
多糖
提取
苯酚-硫酸法
Walnut shell
Polysaccharides
Extraction
Pheno-sulfuric acid method
