摘要
本文使用延迟引出基体辅助激光解吸 /电离质谱 ( DE- MALDI- MS)法 ,以3-羟吡啶甲酸和吡啶甲酸混合液为二元基体 ,硝化纤维膜为基底 ,对混合碱基组成的 DNA样品进行高分辨率测定 ,获得满意的结果 :DNA2 0 - mer分子离子峰分辩率为 8769,DNA 36- mer为 1 650 ,DNA 54- mer为 843,前二者的组成分子离子峰各同位素峰清晰可辩。此外对影响 DNA离子峰分辨率的各种因素进行讨论。
Delayed Extraction Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (DE MALDI MS)was used to high resolution analysis of mix based DNA samples,using the binary matrices 3 HPA(3 Hydroxy 2 Picolinic Acid)/PA(Picolinic Acid)and substrate nitrocellose film.The results were satisfactory:the resolution of DNA 20 mer molecular ion peak was 8769,DNA 36 mer was 1650,and DNA 54 mer was 843.The molecular ion isotope peaks of 20 mer and 36 mer were separated distinctly.We also studied some kinds of factors that affect seriously the resolution of DNA mass spectra.
出处
《质谱学报》
EI
CAS
CSCD
2000年第1期1-6,共6页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金!资助项目 批准号 :2 91 70 2 4 4
