摘要
本文叙述应用自行研制的激光微探针飞行时间质谱仪及采用近年发展起来的基体辅助激光解吸的新方法对多种已知蛋白质的分子量进行测量，表明此法比一般的常规方法具有速度快（１０分钟１个样品）、准确度高（±１％～０．１％）、灵敏度高（１０－１２～１０－１５ｍｏｌ）等优点。同时我们结合ＳＤＳ－ＰＡＧＥ等生化方法，对蛋白质、多肽、伴花生蛋白酶和合成多聚物的分子量进行测定，也取得了令人满意的结果。
Matrix-assisted laser desorption mass spectrometry(MALDI-MS) has been used to determine the molecular weight of proteins with a home-made laser microprobe time offlight mass spectrometer.It demonstrated that this new method is possesed of high accuracy(±1 %￣ 0.1%),high sensitivity(10-12￣10-15mol) and rapidity(with few minutes),which are far better than those traditional methods.By the combination of SDS-PAGE and degradation,we had determined the molecular weight of proteins,polypeptides,conarschin protein and synthesized polymers with satisfied results.
出处
《质谱学报》
EI
CAS
CSCD
1996年第2期1-7,共7页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金
关键词
质谱法
大分子
生物化学
分子量
MALDI-MS
matrix assisted laser desorption mass spectrometry,macromolecule,determination of molecular weight
