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SSH技术在畜禽繁殖研究中的新进展

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摘要 抑制性消减杂交(SSH)技术是一种研究不同细胞(组织)或同类细胞(组织)在不同发育阶段、不同生理状态下差异表达基因的方法,与其他技术相比,该技术具有特异性强、灵敏度高、操作简便和周期短等优点,在畜禽繁殖研究中取得了重要进展。
作者 田勇 卢立志
出处 《畜牧与兽医》 北大核心 2012年第8期84-86,共3页 Animal Husbandry & Veterinary Medicine
基金 国家水禽产业技术体系(CARS-43-2) 浙江省重大科技专项(优先主题)国际科技合作项目(2008C14073) 浙江省科技厅重大科技专项(2007C14023)
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  • 1Diatchenko L, Lau Y F, Campbell A P, et al. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries [ J]. Proc Natl Acad Sci USA, 1996, 93(12): 6025-6030.
  • 2Dai Z M, Zhu X J, Yang W J. Full-length normalization subtractive hybridization: a novel method for generating differentially expressed cDNAs [J]. Mol Bioteehnol, 2009, 43(3) : 257-263.
  • 3Bonnet A, Frappart P O, Dehais P, et al. Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization [J]. Reprod Biol Endocrinol, 2006, 4: 35-45.
  • 4Fayad T, Levesque V, Sirois J, et al. Gene expression profiling of differentially expressed genes in granulosa cells of bovine dominant follicles using suppression subtractive hybridization [ J ]. Biol Reprod, 2004, 70(2) : 523-533.
  • 5Ndiaye K, Fayad T, Silversides D W, et al. Identification of down-regulated messenger RNAs in bovine granulosa cells of dominant fol- licles following stimulation with human chorionic gonadotropin [J]. Biol Reprod, 2005, 73(2): 324-333.
  • 6Baillet A, Mandon-Pepin B, Cabau C, et al. Identification of transcripts involved in meiosis and follicle formation during ovine ovary development [J]. BMC Genomies, 2008, 9: 436-447.
  • 7Baillct A, Le Bouffant R, Volff J N, et al. TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across verte-brates [J]. PLoSOne, 2011, 6(11): e26950.
  • 8Chen A Q, Wang Z G, Xu Z R, et al. Analysis of gene expression in granulosa cells of ovine antral growing follicles using suppressive subtractive hybridization [J]. Anim Reprod Sci, 2009, 115 (1-4) : 39-48.
  • 9郭军,章双杰,邹剑敏,陆火林,苏建东,汤青萍.抑制消减杂交技术筛选鹅产蛋期差异表达基因[J].安徽农业科学,2011,39(8):4710-4713. 被引量:5

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