摘要
目的了解SPF级实验动物中绿脓杆菌(Pseudomonas aeruginosa,PA)感染现状和规律,为防止绿脓杆菌感染提供依据。方法参照国家标准,对SPF级小鼠、大鼠、豚鼠和兔进行检测。结果在2008～2011年中检测的SPF级动物中,PA感染的阳性率为8.9%(267/2988);不同动物感染率无显著差异;不同时间(不同年份和不同季度)PA阳性检出率无显著差异;其中,免疫缺陷动物的PA阳性检出率为32.5%明显高于免疫功能正常动物的7.6%;PA检出率随着送检单位和动物数量的增加逐年升高;不同动物群体间感染率相当,且保持稳定。结论北京地区SPF级实验动物中PA检出率较高,应加强管理。
Objective Understand the SPF laboratory animals Pseudomonas aeruginosa（PA） infection status and regular pattern in order to reduce the pollution of PA provides the basis.Methods According to laboratory animals national standard-Microbiological examination methods of PA,SPF mice,rats,guinea pigs and rabbits were monitored.Results PA positive rate was 8.9%（267/2988） from 2008 to 2011 in total SPF laboratory animals;There was no significant difference in PA infection rates for different species,and no significant difference in infection rates for quarters of different years;There was significant difference in PA infection rates between immunodeficiency animals（32.5%） and normal animals（7.6%）;positive rate of PA with the clients and sample animals increased year by year.PA positive rate was similar and remained stabilization among laboratory animals populations.Conclusions SPF laboratory animals PA positive rate remain high,we should strengthen laboratory animal husbandry,especially immunodeficiency animals in Beijing area
出处
《实验动物科学》
2012年第3期24-26,共3页
Laboratory Animal Science
关键词
绿脓杆菌
实验动物
Pseudomonas aeruginosa
laboratory animal
