摘要
目的观测抗痛风胶囊(AGC)治疗急性痛风性关节炎(AGA)时肿瘤坏死因子(TNF-α)水平的变化,探讨AGC的作用机制。方法大鼠随机分为5组,关节腔内注射尿酸钠制备AGA模型,容积法测定关节容积,ELISA法定量测定血清及关节液中TNF-α含量。结果与模型组比较,高、低剂量的AGC(0.3g.kg-1.d-1和1.2g.kg-1.d-1)和阳性药(秋水仙碱0.8mg/kg)均明显降低AGA大鼠踝关节容积,减少尿酸钠所致关节肿胀程度,明显改善大鼠步态。模型组外周血血清和关节液中TNF-α含量均明显高于空白组,AGC和秋水仙碱均降低AGA大鼠外周血血清和关节液中TNF-α的含量,但AGC组TNF-α水平下降更明显。结论 AGC对大鼠AGA具有明显治疗作用,其机制与降低血循环和关节液的TNF-α水平,减轻消除炎症损伤有关。
Objective To observe and determine the change of TNF-α in acute gouty arthritis（AGA） rat treated with anti-gout capsule,and explored the mechanism of anti-gout capsule.Methods Rats were randomly divided into 5 groups.AGA rat was made by intraarticular injection uric acid sodium.The gait changes were observed,and joint volume were determined by volume method.The content of TNF-α were detected by ELISA in serum and synovial fluid.Histopathological changes were test by HE staining.Expression of TNF-α protein was determined by immunohistochemistry and image analysis.Results Compared with model group,All of low（0.3 g·kg-1·d-1）,high dose（1.2 g·kg-1·d-1） anti-gout capsule and positive control cochicine（0.8 mg/kg） could obviously decreased joint volume,and inhibited ankle swelling caused by uric acid sodium.The content of TNF-α were obviously higher in serum and synovial fluid of AGA rats.The level of serum and synovial fluid TNF-α were lower in anti-gout capsule groups than model group.Conclusion Anti-gout capsule has significantly therapeutic effects for AGA in rat.The mechanism may be related to decreasing the level of TNF-α in blood circulation and local ankle joint,and relieving injury of joint tissue.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第22期2273-2275,共3页
Chongqing medicine
基金
合肥医学院附属医院课题资助项目(院字201009号)
