摘要
目的 探讨胸腺在环磷酰胺 (Cy)增强的小鼠接触性超敏反应 (CHS)中的作用及木瓜苷 (GCS)对胸腺T淋巴细胞亚型的影响。方法 采用了 2 ,4 二硝基氟苯 (DNFB)诱导小鼠CHS模型及Cy诱导小鼠增强CHS模型 ,检测ConA诱导的小鼠脾脏T淋巴细胞增殖、胸腺T淋巴细胞亚型和ConA诱导的胸腺T淋巴细胞培养上清中TGF β1,IL 4和IL 2水平。结果 小鼠CHS模型中 ,ConA诱导的脾淋巴细胞增殖增强 ,CD4 +CD8+双阳性胸腺T淋巴细胞比例增加 ,胸腺细胞产生的Th1和Th3型细胞因子IL 2和TGF β1水平增高而Th2型细胞因子IL 4水平降低。DNFB初次致敏前 3d腹腔注射Cy (2 5 0mg·kg- 1)可以增强CHS反应。GCS(12 0和 2 4 0mg·kg- 1)连续灌胃 12d可以提升Cy增强的小鼠CHS胸腺T淋巴细胞中CD4 - CD8- 和CD4 +CD8- 细胞比例 ,降低CD4 +CD8+细胞比例 ;并提高胸腺淋巴细胞培养上清中IL 4水平 ,降低IL 2和TGF β1水平。结论 GCS对Cy增强的小鼠CHS有明显抑制作用 ;可有效调节小鼠胸腺CD4 /CD8和Th 淋巴细胞亚群及细胞因子产生平衡。
AIM To investigate the roles of thymus T lymphocytes subsets i n contact hypersensitivity (CHS) and effects of glucosides of Chaenomeles speciosa (GCS) on this response. METHODS CHS model induced by 2,4-dinitro-I-dinitrofluorobenzene (DNFB) and cyclophosphamide (Cy) potentia t ed CHS model were used. GCS(60,120 and 240 mg·kg -1) were given intragastr ically (ig) once daily for 12 consecutive days. Concanavalin A (Con A)-induced lymphocytes proliferation was observed by MTT assay. CD4/CD8 T lymphocytes subse ts were measured by flow cytometry and the levels of Con A-induced cytokines fr om thymocytes were measured by enzyme-linked immunosorbent asssay (ELISA). RESULTS Splenocyte proliferation stimulated by Con A was augmented and CD4+CD8+ T lymphocytes were increased in thymus of mice with CHS. The b alance of CD4+T h subsets was changed to favor T h1 and T h3 as shown by th e increased interleukin-2 (IL-2) and transforming growth factor β 1 (TGF-β 1) production and the decreased interleukin-4 (IL-4) production. GCS, simila r as the control drug actarit (4-acetylaminophenylacetic acid), could elevate t he percentage of CD4+CD8- T lymphocytes and CD4-CD8- T lymphocytes, and reduce the percentage of CD4+CD8+ T cells in Cy-potentiated CHS mice. GCS i nhibited the production of IL-2 and TGF-β 1, and increased the IL-4 level i n cultures of thymocytes from mice with Cy-potentiated CHS. CONCLUSION GCS inhibits Cy-potentiated mice CHS response and modulates the balance of CD4/CD8 or helper T cells subsets.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2004年第6期415-420,共6页
Chinese Journal of Pharmacology and Toxicology
基金
安徽省自然科学基金资助项目 (0 0 0 4 4 4 11)~~
