摘要
以甘肃河西走廊具有代表性的三个产地的啤酒大麦为研究对象,通过对大麦表面细菌、酵母菌和霉菌的筛查、分离纯化与鉴定,研究大麦附生微生物区系组成及其优势种群。结果显示,河西走廊啤酒大麦附生细菌数量最多,菌落总数在106～107cfu/g之间,酵母总数在104～105cfu/g之间,霉菌菌落总数因产地变化较大,数量在103～105cfu/g。通过对大麦附生微生物区系的鉴定,分离到9株优势细菌,9株优势酵母和11株霉菌,表明大麦附生菌群具有一定的多样性,并因产地不同,微生物组成呈现出一定差异。部分样品大麦中检出了产气肠杆菌(Enterobacter aerogenes)和产色葡萄球菌(Staphylococcus chromogenes)等细菌,以及一些产真菌毒素霉菌菌株,存在一定生物安全隐患。建议在制麦过程中,尤其是浸麦工段应加强洗麦,以减少原料大麦携带的有害病菌,并降低产生真菌毒素的风险。
In this experiment,three typical beer barley yielded from different areas in Gansu Corridor were selected in order to determine its epiphytic microbial flora and the dominant microbe species.The results showed that the epiphytic bacteria colony count on barley was the largest,reached 106 ～ 107cfu/g;the yeast count was 104 ～ 105cfu/g;and the mould count was 103 ～ 105cfu/g,which vary greatly between different origins.Nine dominate strains of bacteria,nine dominate strains of yeasts and eleven dominate strains of moulds had been separated and identified from epiphytic micro bial flora of barley.It indicated that epiphytic microbial flora of barley had certain diversity,and there were some differences in microbial composition come from different origins.Enterobacter aerogenes,Staphylococcus chromogenes and some mould strains that probably produce mycotoxins were detected out from a few of samples.This inferred that there existed a biological safety problem.Therefore barley cleaning should be strengthened during malting,especially steeping stage so as to reduce the harmful pathogens carried by barley.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第15期62-66,70,共6页
Science and Technology of Food Industry
基金
甘肃省自然科学研究基金计划(1107RJZA128)
甘肃省科技重大专项计划(1002NKDH029)
关键词
河西走廊
啤酒大麦
附生微生物
区系
Gansu Corridor
beer barley
epiphytic microorganism
flora
