摘要
采用碳化二亚胺法将头孢噻肟钠(CTX)与牛血清白蛋白(BSA)以及卵清白蛋白(OVA)分别进行偶联,合成完全抗原(CTX-BSA,CTX-OVA),紫外吸收光谱(UV)和凝胶电泳(SDS-PAGE)进行鉴定并测定CTX-BSA的偶联比,结果显示:与CTX(235.0 nm)、BSA(278 nm)、OVA(232.0,278.0 nm)相比,CTX-BSA(213.0,275.5 nm),CTX-OVA(223.5,274.5 nm)特征吸收峰位移明显,CTX-BSA的偶联比为5.8∶1.CTX-BSA与BSA以及CTX-OVA与OVA的SDS-PEG图谱相比,完全抗原泳动速度滞后.以CTX-BSA免疫动物并用间接酶联免疫吸附法(ELISA)测定抗血清效价,血清效价≥1∶100000.以CTX对免疫血清进行间接竞争ELISA测定,CTX可有效抑制免疫血清效价.
Cefotaxime Sodium （CTX） was linked to bovine serum albumin （BSA） and ovalbumin （ OVA）, and was used to synthesize complete antigen CTX-BSA, CTX-OVA by carbodiimide method respectively. Using ul- traviolet absorption spectrum and gel electrophoresis to identify complete antigen and test coupling ratio. The results showed that the displacement of characteristic absorption peak shifted obviou：sly, compared CTX-BSA （213.0 nm,275.5 nm ）,CTX-OVA（223.5 nm,274.5 nm） with CTX（235.0 nm）,BSA（278 nm）,OVA （232.0 nm,278.0 nm） according to ultraviolet absorption spectrum. The coupling ra｜Jo of CTX-BSA was 5.8 ： 1. The atlas of gel electrophoresis displayed that CTX-BSA moved slowly than BSA did, so did CTX-OVA. The serum valence immunized by CTX-BSA can reach to 1：100000 by using indirect ELISA detection. The indirect competitive ELISA serum inhibition test illustrated that the serum characterized with the antigen-specific anti- body response to CTX can clearly inhibit the serum titer.
出处
《鲁东大学学报（自然科学版）》
2012年第3期242-247,共6页
Journal of Ludong University:Natural Science Edition
基金
山东省星火计划(2011SH05012)
