摘要
目的探讨脑梗死大鼠外周细胞免疫功能改变与脾脏质量指数和组织学变化的关系。方法成年雄性大鼠制作大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）模型，检测外周血细胞因子水平、脾脏质量指数和组织学变化。结果大鼠血清促炎细胞因子白细胞介素（interleukin，IL）-1β和干扰素（interferon，INF）-γ水平分别在模型制作后6h和12h升高，24h时开始回落，72h时降至最低，较假手术组存在显著统计学差异（P〈0．01）；相反，抗炎细胞因子IL-10水平则在模型制作后6h时降低，12h时回升，72h时达高峰，较假手术组存在显著统计学差异（P〈0．01）。MCAO组大鼠脾脏质量指数在模型制作后6h显著降低（P〈0．01），12h时回升，但仍低于假手术组（P〈0．01），随后逐渐下降，72h时降至最低（P〈0．01）。HE染色显示，MCAO后72h大鼠脾脏的生发中心明显缩小，且轮廓显示欠清晰。相关分析显示，促炎细胞因子IL-1β（r=0．304，P=0．002）和INF-γ水平（r=0．644，P=0．000）与脾脏质量指数均呈显著正相关，而抗炎性细胞因子IL-10水平与脾脏质量指数呈显著负相关（r=-0．492，P=0．000）。结论MCAO大鼠外周细胞免疫功能处于抑制状态，脾脏变化可能在卒中后免疫抑制的发生过程中起着重要作用。
Objective To investigate the relationship between the changes of peripheral cellular irranune function and the spleen weight index and the spleen histopathological changes following cerebral ischemia- reperfusion in rats. Methods A model of middle cerebral artery occlusion （MCAO） of adult male rats was induced. The peripheral blood cytokine levels, spleen weight index and the spleen histopathological changgs were evaluated. Results The serum proinflanamtory cytokine interleukin （IL） -1β and interferon （1NF） -γ levels in rats increased at 6 and 12 hours respectively after modeling. They began to decrease at 24 hours and decreased to the lowest at 72 hours. Compared to the sham operation group, there were sigtificant differences （P 〈0. 01）; on the contrary, the anti-inflammatory cytokine IL-10 level decreased at 6 hours after modeling. It began to increase at 12 hours and reached the peak at 72 hours. Compared to the sham operation group, there were significant differences （P 〈0. 01）. The spleen weight index in a MCAO group decreased significantly at 6 hours after modeling （P 〈 0. 01） and it began to increase at 12 hours, but it was still lower than that in the sham operation group （P 〈0. 01）, and then it decreased gradually and decreased to the lowest at 72 hours （P 〈0. 01）. HE staining showed that the spleen gmninal centers of rats reduced sigaificantly at 72 hours after MCAO and the contours did not show very clear. The correlation analysis showed that the proinflammatory cytokine IL-1β （r =0. 304, P =0. 002） and INF-γ（r =0. 644, P =0. 000） levels were positive correlated with the spleen weight index, and the anti-inflanmmtory cytokine IL-10 level was negatively correlated with the spleen weight index （r = 0. 492, P = 0. 000）. Conclusions The rat peripheral cellular immune function after MCAO was in an inhibitory state. The changes of spleen may play an important role in the process of immunosuppression after stroke.
出处
《国际脑血管病杂志》
北大核心
2012年第6期418-422,共5页
International Journal of Cerebrovascular Diseases
基金
卫生部科研基金（LW201007）
关键词
脑缺血
再灌注损伤
细胞因子
脾脏
疾病模型
动物
大鼠
Brain Ischemia
Reperfiasion Injury
Cytokines
Spleen
Disease Models, Animal
Rats
