摘要
目的探讨脾切除对脑缺血大鼠脑梗死体积的影响及其可能机制。方法18只雄性Sprague—Dawley大鼠随机分为脾切除组、假脾切除术组和对照组，每组6只。脾切除2周后，采用线栓法建立大鼠大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）模型，24h后灌注固定并取脑。尼氏染色法测定脑梗死体积，免疫荧光染色法检测缺血皮质巨噬细胞数量。结果脾切除组（34．93％±3．23％）脑梗死体积显著小于假脾切除组（74．33％±2．36％，q=39．399，P〈0．001）和对照组（77．30％±2．62％，q=42．369，P〈0．001），但假脾切除组与对照组之间无显著差异（q=2．970，P=0．082）。脾切除组缺血皮质巨噬细胞数量[（3．4±1．07）个腐倍镜视野]显著少于假脾切除组[（20．7±4．37）个／N倍镜视野，q=17．300，P〈0．001）和对照组[（18．87±4．17个）扁倍镜视野（q=15．467，P〈0．001]；但假脾切除组与对照组无显著差异（q=1．833，P=0．384）。结论脾切除可能通过减少脑缺血皮质区巨噬细胞数量缩小脑梗死体积。
Objective To investigate the effect of splenectomy on infarct volume in middle cerebral artery occlusion in focal cerebral ischemia rats and its possible mechanisms. Methods Eighteen male Sprague-Dawley rats were randomly divided into splenectomy, sham splenectomy, and control groups （n =6 in each group）. A model of middle cerebral artery occlusion （MCAO） was induced by the intraluminal suture method 2 weeks after splenectomy. The rats were decapitated and their brains were removed after 24 hours. The infarct volume was measured with Nissl body staining The number of macrophages in ischernic cortex was detected with immuno- fluorescence staining. Results The infarct volume in the splenectomy group （34. 93% ± 3.23% ） was significantly smaller than that in the sham sp!enectomy group （74. 33%±2.36%; q = 39. 399, P 〈 0. 001 ） and the control group （77. 30% ± 2.62% ; q = 42. 369, P 〈 0. 001 ）. However, there was no significant difference between the sham splenectomy group and the control group （q =2. 970, P =0. 082）. The number of macrophages of the ischemic cortex in the splenectomy group （3.4 ±1.07/per high power field） was significantly less than that in the sham splenectomy group （20. 7 ±4. 37/per high power field; q = 17. 300, P 〈 0. 001 ） and the control group （18. 87 ±4. 17/per high power field; q = 15.467, P 〈0. 001）. However, there was no significant difference between the sham splenectomy group and the control group （q = 1. 833, P =0. 384）. Conclusions Splenectomy may reduce the infarct volume by reducing the number of macrophages in ischemic cortical region.
出处
《国际脑血管病杂志》
北大核心
2011年第9期663-666,共4页
International Journal of Cerebrovascular Diseases
基金
国家自然科学基金（30971027）
关键词
脑缺血
脾切除术
脾
巨噬细胞
炎症
大鼠
Brain ischemia
Splenectomy
Spleen
Macrophages
Inflammation
Rats
