摘要
目的:研究不同外界因子对粘毛黄芩毛状根生长和黄酮类化合物合成的影响.方法:研究不同基本培养基、pH值、碳源及激素对粘毛黄芩毛状根生长和黄酮类化合物合成的影响.结果:30g.L-1蔗糖、pH 5.8的1/2MS培养基最适合毛状根生长,但黄酮类化合物合成最高时pH值为6.4.外源激素NAA、GA对毛状根的生长有促进作用,GA效果最为明显.结论:初步优化了粘毛黄芩毛状根的培养条件,为实现粘毛黄芩黄酮类化合物大规模生产奠定了基础.
Objective：To study the effects of different physical and chemical factors on hairy root growth and flavonoids production in suspension culture of Scutellaria viseidula Bge hairy root. Methods ： To study hairy root growth and flavonoids production in factors of the media used,pH values,sources of carbon and exogenous phytohormones. Results： The best conditions of hairy root culture were 1/2MS medium with sucrose （3%） as carbon material and pH 5.8, but the optimum pH for fla-vonoids production was 6.4; NAA and GA could improve the growth speed of the hairy root,while the most obvious effect is GA. Conclusion： Eminent Cultured-conditions of hairy root give a new pathway to resolve the shortage of baicalin of Scutellaria viscidula Bunge.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期124-126,共3页
Journal of Henan Normal University(Natural Science Edition)
基金
合肥市科技局资助项目(201016)
关键词
粘毛黄芩
毛状根
黄酮类化合物
Scutellaria viscidula Bge
hairy root
flavonoids
