摘要
目的：探讨建立适合微波消融研究的肝癌小鼠模型。方法：将C57BL／6J小鼠随机分为4组（n=10），分别皮下注射不同数量（5×10^5，1×10^6，2×10^6，5×10^6）的Hepal-6细胞，比较各组成瘤率；选择成瘤率理想的细胞数再次造模，待瘤体长径达8-10mm时将模型小鼠随机分为4组（n=10），对瘤体行微波热消融处理，瘤体边缘温度分别控制在60℃，55℃，50℃和45℃，消融时间为3min，比较各组完全消融率及消融前后的瘤体病理变化。结果：2×1×10^6的Hepal-6细胞皮下注射组成瘤率高且适合消融操作；60℃消融组出现组织碳化，其余各组比较，55℃消融组完全消融率最高，复发率最低（P〈0．01）。结论：对小鼠Hepal-6细胞皮下移植性肝癌模型行55℃×3min的微波热消融有效、可行。
Objective： To establish a mice model of liver cancer suitable for microwave ablation.
Methods： C57BL/6J mice were randomly divided into 4 groups （n=10 in each group） and subcutaneously injected with Hepal-6 cells of different numbers （5×10^6, 1×10^6, 2×10^6 and 5×10^6） respectively, and then the tartor formation rates in the 4 groups were compared. Next, the ideal cell number for tumor formation was selected to establish the mouse models once again. When mean tumor diameter reached 8-10 mm, the tumor-bearing mice were randomly divided into 4 groups （n=10 in each group） to undergo microwave thermal tumor ablation. The temperature at the edge of the tumor was maintained at 60, 55, 50, and 45 % respectively, and the ablation time was 3 min. The complete ablation rate and pathological changes of tumor among the 4 groups were compared.Results： The group with 2×10^6 Hepal-6 subcutaneous injection had a high tumor formation rate and was suitable for ablation. Except for 60% ablation group, in other 3 groups, the 55℃ablation group had the highest complete ablation rate and the lowest recurrence compared with the other two groups （P〈0.05）. Conclusion： 55℃ × 3 min microwave ablation is feasible and effective for Hepal-6 cells subcutaneously implanted model of liver cancer in mice.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第8期973-977,共5页
China Journal of General Surgery
基金
国家自然科学基金资助项目(81071892)
广州市医药卫生科技项目(20121A010012)
高等高校博士学科点专科专项科研基金(新教师类
201000171120072)
高校基本科研业务费中山大学青年教师培育项目(09ykpy30)
关键词
肝肿瘤
实验性
热消融
疾病模型
动物
小鼠
Liver Neoplasms, Experimental
Thermal Ablation
Disease Models, Animal
Mice
