摘要
背景:以天然可吸收的壳聚糖为膜载体,加入具有抗菌消炎、促进创面愈合的槲皮素制成复合膜,拟通过动物模型对该膜的药理作用进行初步观察。目的:观察槲皮素壳聚糖复合药膜对NaOH所致大鼠口腔溃疡的治疗作用。方法:采用NaOH烧灼法制备SD大鼠口腔溃疡模型80只,随机分为4组,空白对照组不采取任何处理措施,壳聚糖膜组在溃疡处贴壳聚糖膜,复合膜组在溃疡处贴槲皮素与壳聚糖复合药膜,冰硼散组在溃疡处喷洒冰硼散,2次/d,直至溃疡完全愈合为止。另外从上述实验动物中取SD大鼠10只,在大鼠背部脊柱两侧制作全层皮肤缺损模型,创面制作后次日,空白对照组不做任何处理,冰硼散组创面涂抹冰硼散液,壳聚糖组创面涂抹壳聚糖液,复合溶液组创面涂抹槲皮素壳聚糖液,2次/d,连续给药3d。结果与结论:复合膜组各时间点的溃疡面积均小于空白对照组(P<0.01),且给药后第2,4天的溃疡面积也小于冰硼散组(P<0.05)。在溃疡表面感染程度上复合膜组明显好于空白对照组(P<0.01),与冰硼散组之间差异无显著性意义。复合溶液组创面组织中超氧化物歧化酶活力明显高于空白对照组(P<0.01),而丙二醛含量低于空白对照组(P<0.01)。结果可见槲皮素壳聚糖复合药膜可促进溃疡面愈合,这可能与其提高创面组织中超氧化物歧化酶活性,降低丙二醛含量有关。
BACKGROUND： Quercetin chitosan composite film was prepared by natural absorbable chitosan and quercetin which have anti-inflammation and accelerating effects on wound healing, and natural absorbable chitosan served as film carriers. A primary observation through an animal model on the pharmacological functions of the composite film was done. OBJECTIVE： To investigate the therapeutic effects of quercetin chitosan composite film on oral ulcer in rats induced by NaOH. METHODS： A SD rat model of oral ulcer induced by NaOH was established. A total of 80 SD rats were randomly divided into four groups： composite film group was given quercetin chitosan composite film, chitosan film group was given chitosan film, Bingpeng San group was given Bingpeng San and control group received no treatment. Each group was dosed twice a day until the oral ulcer in rats was healed completely. Besides, 10 rats from each group were selected, and a rat model of full-thickness back skin defects on both sides of the spine was established. On day 2 after the model was established, the composite film group was treated with quercetin chitosan solution; the chitosan film group was treated with chitosan solution; the Bingpeng San group was smeared with Bingpeng San solution and the control group underwent no treatments. These groups were dosed twice a day for 3 days. RESULTS AND CONCLUSION： Compared with the control group, the ulcer area of the composite film group at each time point was smaller （P 〈 0.01 ）. At days 2 and 4 after administration, the ulcer area of the composite film group was also smaller than that of the Bingpeng San group （P 〈 0.05）. The composite film group was obviously better than the control group at the degrees of ulcer infection （P 〈 0.01 ）. There was no significant difference between the composite film group and Bingpeng San group. The superoxide dismutase activity in the wound tissue of rats in the composite film group was significantly higher than that in the control group （P 〈 0.01 ）, while malondialdehyde content in the composite film group was lower than that in the control group （P 〈 0.01 ）. These findings suggest that quercetin chitosan composite film can promote ulcer wound healing, which may be related with the increase of superoxide dismutase activity and the decrease of malondialdehyde content in the wound tissue.
出处
《中国组织工程研究》
CAS
CSCD
2012年第29期5437-5440,共4页
Chinese Journal of Tissue Engineering Research
基金
河北省自然科学基金项目(C2010001795)
河北省唐山市科技攻关项目(11150205A-8)
河北联合大学青年基金项目(Z201134)~~
