期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

猪圆环病毒2型10JS-2株的分离与全基因组序列分析 被引量：4

Isolation and Complete Genome Sequence Analysis of Porcine Circovirus Type 2(PCV2) Strain 10JS-2 from Jiangsu Province,China
在线阅读 下载PDF
导出
摘要 本试验从江苏某猪场采集猪血清,进行猪圆环病毒2型(PCV2)的检测和分离,根据GenBank中登录的PCV2全基因组序列,设计1对特异性引物,扩增PCV2全基因组,并且进行序列测定和分析。发现一株PCV2 10JS-2的基因组全长为1779nt,在病毒复制起始区域含有11个碱基的插入。在GenBank中对含有11个碱基插入的毒株序列进行BLAST发现,有两株PCV2(AY321993和EF565360)也有11个碱基的插入,但是与10JS-2比较,插入的碱基和插入的位置不同。遗传进化分析显示10JS-2属于PCV2a基因型,AY321993和EF565360属于PCV2b基因型,因此推断10JS-2可能是由PCV2a基因型的毒株在复制起始区域发生碱基插入形成的。这是首次发现此类PCV2毒株。 Serum sample was collected from a pig in Jiangsu province,China, and virus detection and isolation of porcine circovirus type 2 （PCV2） were performed. A pair of primers was designed to amply the complete PCV2 genome according the PCV2 sequences submitted to GenBank for sequencing and analysis. A PCV2 strain 10JS-2 was isolated. The complete genomic sequence of 10JS-2 was 1779 nt in length. Remarkably,a special 11 nt insertion in the origin of viral genome replication （Ori） of 10JS-2 was observed. BLAST search on NCBI website revealed that another two PCV2 strains （AY321993 and EF565360） also had 11 nt insertion in Ori. However,the insertion sequence and the location of the insertion were different. Phylogenetic analysis revealed that 10JS^2 belong to genotype PCV2a,while AY321993 and EF565360 were classified into genotype PCV2b. So, we inferred that 10JS-2 probably derived from PCV2a strains that obtained the 11 nt insertion in Ori. This is the first report about this kind of PCV2a strain.
作者 蔡林 胡冬梅 李晓霞 汪葆玥 韩雪 倪建强 周智 遇秀玲 翟新验 王文良 田克恭
机构地区 中国动物疫病预防控制中心 军事医学科学院
出处 《中国畜牧兽医》 CAS 北大核心 2012年第7期6-10,共5页 China Animal Husbandry & Veterinary Medicine
基金 北京市生猪产业技术体系创新团队(GWZJ-2009-05)
关键词 猪圆环病毒2型 分离 全基因组序列 序列分析 porcine circovirus type 2 isolation complete genome sequence sequence analysis
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献17

  • 1Balmelli C,Steiner E,Moulin H,et al. Porcine circovirus type 2 DNA influences cytoskeleton rearrangements in plasmacytoid and monocyte-derived dendritic cells[J]. Immunology,2011,132: 57~65.
  • 2Cino-Ozuna A G,Henry S,Hesse R,et al. Characterization of a new disease syndrome associated with porcine circovirus type 2 in previously vaccinated herds[J]. J Clin Microbiol,2011,49: 2012~2016.
  • 3Cortey M,Olvera A,Grau-Roma L,et al. Further comments on porcine circovirus type 2 (PCV2) genotype definition and nomenclature[J]. Vet Microbiol,2011,149: 522~523.
  • 4de Boisseson C,Beven V,Bigarre L,et al. Molecular characterization of porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs[J]. J Gen Virol,2004,85: 293~304.
  • 5Dupont K,Nielsen E O,Baekbo P,et al. Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time[J]. Vet Microbiol,2008,128: 56~64.
  • 6Finsterbusch T,Mankertz A. Porcine circoviruses--small but powerful[J]. Virus Res,2009,143:177~183.
  • 7Gillespie J,Opriessnig T,Meng X J,et al. Porcine circovirus type 2 and porcine circovirus-associated disease[J]. J Vet Intern Med,2009,23: 1151~1163.
  • 8Grau-Roma L,Fraile L,Segales J. Recent advances in the epidemiology,diagnosis and control of diseases caused by porcine circovirus type 2[J]. Vet J,2011,187: 23~32.
  • 9Lu G,Moriyama E N. Vector NTI,a balanced all-in-one sequence analysis suite[J]. Brief Bioinform,2004,5: 378~388.
  • 10Ma C M,Hon C C,Lam T Y,et al. Evidence for recombination in natural populations of porcine circovirus type 2 in Hong Kong and China's Mainland[J]. J Gen Virol,2007,88: 1733~1737.

同被引文献36

  • 1Allan G M, McNeilly F, Ellis J, et al. Experimental infection of colostrums deprived piglets with porcine cireivirus 2 (PCV2)and porcine reproductive and respiratory syndrome virus (PRRSV)potentiates PCV2 replication E J ]. Arch Virol, 2000, 145 ( 11 ) : 2421-2429.
  • 2Dupont K, Nielsen E O, Baekbo P, et al. Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case- control study supports a shift in genotypes with time[J]. Vet Mi- crobiol,2008,128(1) - 56-64.
  • 3Gagnon C A,Tremblay D,Tijssen P,et al. The emergence of por- cine circovirus 2b genotype (PCV-2b) in swine in Canada[J]. Can Vet J,2007,48(8) :811-819.
  • 4Guo L J, Lu Y H, Wei Y W, et al. Porcine circovirus type 2 (PCV2): Genetic variation and newly emerging genotypes in China[J]. Viology Journal, 2010,7 (1) : 273 - 284.
  • 5Lefebvre D J, Costers S, Van Doorsselaere J, et al. Antigenic differences among porcine circovirus type 2 strains, as demonstra- ted by the use of monoclonal antibodies[J]. J Gen Virol,2008,89 (1) :177-187.
  • 6Meehan B M, McNeilly F, McNair L, et al. Isolation and charac- terization of porcine circovirus 2 from cases of sow abortion and porcine dermatitis and nephropathy syndrome[J]. Aehives of Vi- rology, 2001,146 (4) : 835 - 842.
  • 7Nielsen J,Vincent I E,Btner A,et al. Association of lymphope-nia with porcine circovirus type 2 induced postweaning multisys- temie wasting syndrome(PMWS) [J]. Vet Immunopathol, 2003, 92(3) :97-111.
  • 8Olvera A, Cortey M, Segales J. Molecular evolution of porcine circovirus type 2 genomes- Phylogeny and clonality[J]. Virolo gy, 2008,357(2) :175-185.
  • 9Sega| S J, Olvera J,Grau-Roma L, et al. PCV2 genotype definition and nomenclature[J]. Vet Rec,2008,162(2) :867-868.
  • 10Stevenson G W,Kiupel M, Mittal S K, et al. T1 distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors[J]. J Vet Diagn Invest,2001,13(1) :57-62.

引证文献4

二级引证文献10

中国畜牧兽医
2012年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部