摘要
目的 :探讨癌基因及抑癌基因改变与膀胱癌生物学行为的关系。方法 :采用免疫组织化学方法及分子生物学技术 ,检测了82例膀胱移行细胞癌P21、P185、P53蛋白表达及38例P53基因突变。结果 :P21、P185、P53蛋白表达阳性率分别为62.2 %(51例)、58.5 %(48例)、31.7 %(26例)。12例(31.6 %)有P53抑癌基因突变 ,其中全部有第280位密码子G C突变 ;3例合并有248密码子突变 ;1例有249密码子点突变。结论 :P21、P53蛋白表达阳性率与膀胱移行细胞癌病理分级及临床分期呈正相关(P<0.01) ;P185蛋白阳性率与分级、分期呈负相关(P<0.05)。P53抑癌基因点突变与病理分级和临床分期呈显著正相关(P<0.05) ;与预后显著相关(P<0.05) ;且死亡率高于点突变阴性者。
Objective: To investigate the relationship between changes of oncogenes and antioncogenes and biological behaviors of cystic carcinoma. Methods: The expression of P21, P185 and P53 and mutation in P53 gene were studied in 82 cases of cystic transitional cell carcinoma by immunohistochemical and molecular biological methods. Results: The positive rates of P21, P53 and P185 were 62.2%, 31.7% and 58.5% respectively. The rates of P21 and P53 were increased with the progression of the pathological grade and the clinical stage (P<0.01) while that of the P185 decreased with them (P<0.05). The point mutation rate in P53 gene was 31.6%. All the P53 point mutation positive cases had G C transition at codon 280. Point mutation at codons 248 and 249 were detected in 3 and 1 respectively among the 12 P53 point mutation positive cases. Conclusion: The positive expression of p21 and p53 protein was positively correlated to the pathological grading and clinical staging of transitional cell carcinoma (P<0.01) while that of p185 was negatively correlated to the grading and staging of the carcinoma (P<0.05). The point mutation of P53 antioncogene is closely related to the high grade and/or high stage subsets of bladder cancer. The point mutation positive cases have a higher recurrent rate and death rate.
出处
《第三军医大学学报》
CSCD
北大核心
2000年第2期126-128,共3页
Journal of Third Military Medical University
