摘要
以竹叶黄酮水提溶液为原料,采用PEG(聚乙二醇)/(NH4)2SO4双水相体系对竹叶黄酮进行萃取,考察了PEG平均相对分子质量、PEG质量分数、(NH4)2SO4质量分数、pH值、NaCl质量分数、原液质量分数、萃取温度等对双水相及竹叶黄酮萃取效果的影响。双水相萃取法提取竹叶黄酮的最优条件为:PEG 400 31%,(NH4)2SO411%,pH 3.9,NaCl 0.7%,原液51.5%,萃取温度20℃,在此条件下得到的竹叶黄酮萃取率为97.8%。结果说明,双水相萃取法操作简单方便,成本低,不会引起生物质失活或变性,适合于黄酮类化合物的萃取分离。
The polyethylene glycol（PEG）/（NH4 ）2 SO4 aqueous two-phase system was used to extract flavonoids from water extract of bamboo leaves. Effects of the molecular weight, mass fraction of PEG, mass fraction of （NH4）2 SO4 , pH, mass fraction of NaC1, mass fraction of water extract solution and temperature on the aqueous two- phase system and extraction of flavonoids were studied. The optimal conditions were obtained by response surface methodology designed as： average molecular weight of PEG was 400, quality percentage of PEG was 31%, quality percentage of （NH4）2 SO4 was 11 ~, pH was 3.9, quality percentage of NaC1 was 0.7 ~, temperature was 20 ~C, qual- ity percentage of water extract solution was 51. 5%. Under these conditions, the extraction rate of flavonoids was 97.8%. The results indicate that aqueous two-phase system is simple and convenient, low cost, without causing inactivation and denaturation of biological materials. The method is suitable for extraction and separation of flavonoids.
出处
《氨基酸和生物资源》
CAS
2012年第2期24-28,共5页
Amino Acids & Biotic Resources
基金
浙江省重点科技创新团队项目(2010R50032)
关键词
竹叶黄酮
双水相萃取
双水相体系
曲面响应法
flavonoids from bamboo leaves
ATPE（aqueous two-phase extraction）
aqueous two-phase system
RSM（response surface methodology）
