摘要
【目的】研究大豆卵磷脂(soya lecithine,SL)对子宫内发育迟缓(intrauterine growth retardation,IUGR)猪肠道生长、黏膜氧化应激(oxidative stress,OS)和热休克蛋白70(heat shock protein,HSP70)表达的影响。【方法】试验共选用12头7 d IUGR仔猪和6头正常体重(NBW)仔猪,所有IUGR仔猪随机分成两组(n=6),分别饲喂基础人工乳(IUGR组)和添加1.5%SL的人工乳(IUGR+SL组),所有NBW仔猪饲喂基础人工乳(NBW组,n=6),试验期7 d。【结果】IUGR显著降低14 d仔猪空肠及其非黏膜绝对重量(P<0.05),降低空肠黏膜总抗氧化能力(T-AOC)、谷胱甘肽还原酶(GR)和谷胱甘肽过氧化物酶(GPx)活性(P<0.05),升高MDA水平(P<0.05),黏膜HSP70的ELISA和免疫印迹法检测结果均显著升高(P<0.05)。IUGR猪补充SL后,空肠肠段、黏膜和非黏膜绝对和相对重量均显著升高(P<0.05);空肠黏膜T-AOC、GPx和SOD显著升高(P<0.05),而MDA显著下降(P<0.05);ELISA检测结果显示HSP70蛋白水平显著降低(P<0.05),且与SL添加水平呈显著负相关(P<0.05)。【结论】补充SL对恢复IUGR导致的仔猪肠道组织生长缓慢、HSP70表达升高和OS损伤有很好的效果。
[ Objective ] The effects of supplement of soya lecithine （SL） on intestinal growth, mucosal heat shock protein 70 （HSP70） expression and oxidation stress （OS） in intrauterine growth retardation （IUGR） piglets were studied. [Method] Twelve IUGR piglets and six piglets with normal birth weight were selected. All piglets were weaned on 7th d of age, and assigned equally to three groups： six NBW and six IUGR piglets were fed with control diet （IUGR）, and six IUGR piglets were fed with diet supplemented with 1.5% SL （IUGR＋SL） for 7 days. [Result] IUGR decreased （P〈0.05） the absolute weigh of jejunal segment and non-mucosa, reduced （P〈0.05） the mucosal capacity of total anti-oxidation （T-AOC）, as well as the activity of GR and GPx, and elevated （P〈0.05） the content of MDA. The mucosal I4SP70 contents detected by ELISA and Western Blot were increased （P〈0.05） by IUGR. After SL treatment, the absolute and relative weight of jejunum, jejunal mucosa and non-muzosa increased （P〈0.05） in IUGR piglets. Supplement of SL increased （P〈0.05） the mucosal T-AOC, GPx and SOD, as well as reduced （P〈0.05） MDA and HSP70 content in terms of ELISA. Inverse correlations were found between SL supplementation in IUGR piglets and HSP70 contents （P〈0.05）. [ Conclusion ] SL plays an important role in recovering impaired jejunal growth, increased mucosal HSP70, and OS induced by IUGR.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第13期2711-2717,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30771569
30972116)
南京农业大学青年科技创新基金(KJ08014)
