摘要
目的探讨益气化瘀化痰法对肺纤维化大鼠肺组织转化生长因子-β1(TGF-β1)及纤溶酶原激活物抑制物-1(PAI-1)的影响。方法采用气管内一次性注射博来霉素5mg/kg诱导生成肺纤维化大鼠模型。将105只SD大鼠分为:正常对照组、模型组、益气组、化瘀组、化痰组、益气化瘀化痰组、激素组。正常对照组和模型组采用生理盐水灌胃,其余各组于造模后28d分别给予益气汤、化瘀汤、化痰汤、益气化瘀化痰汤30g.kg-1.d-1灌胃,激素组给予皮下注射氢化可的松20mg.kg-1.d-1干预,2个月后处死各组大鼠,ELISA法检测各组大鼠血浆PAI-1含量,Western blot法检测各组大鼠肺组织TGF-β1蛋白表达。结果益气组、化瘀组、化痰组及益气化瘀化痰组大鼠血浆PAI-1含量水平较模型组均降低(P<0.05),其中益气化瘀化痰组降低最显著(P<0.05);益气组、化瘀组、化痰组及益气化瘀化痰组大鼠肺组织TGF-β1蛋白表达较模型组均减弱(P<0.05),尤以益气化瘀化痰组减弱最明显(P<0.05)。结论益气、化瘀、化痰法能显著改善博来霉素所致大鼠肺纤维化的程度,其机制可能与抑制TGF-β1及PAI-1的表达有关。
Objective To evaluate the effects of therapeutics of benefiting qi,expelling blood stasis and dissipating phlegm on transforming growth facto-β1 （TGF-β1）and plasminogen activator inhibitor-1 （PAI-1） of rats with pulmonary fibrosis. Methods We built the model of pulmonary fibrosis in rats by disposable endotracheal injection of bleomycin 5 mg/kg. 105 SD rats were divided into 7 groups, including normal group, model group, the benefiting qi group, the expelling blood stasis group, the dissipating phlegm group,the benefiting qi, expelling blood stasis and dissipating phlegm group, hormone group. Normal control group and model group were lavaged with normal saline, hormone group was given subcutaneous injection of hydrocortisone 20 mg·kg-1 , the rest groups were lavaged with corresponding medicines 30 g ·kg-1 ·d-1 （the benefiting qi decoction, the expelling blood stasis decoction,the dissipating phlegm decoction, the benefiting qi, expelling blood stasis and dissipating phlegm decoction） separately for 28 d after pulmonary fibrosis models were established. All rats were killed after 2 months＇ interventing treatment. We measured plasma PAI-1 levels in each group by ELISA,and detected the lung tissue expression of TGF-~I protein by Western blot. Results PAI-1 contents in plasma of rats in the benefiting qi group, the expelling blood stasis group, the dissipating phlegm group, the benefiting qi, expelling blood stasis and dissipating phlegm group were lower than those of the model group （P〈0.05）, especially the benefiting qi,expelling blood stasis and dissipating phlegm group reduced most obviously（P〈0.05）. TGF-β1 protein expressions in the benefiting qi group,the expelling blood stasis group, the dissipating phlegm group, the benefiting qi, expelling blood stasis and dissipating phlegm group were weakener than those in the model group（P〈0.05） ,the benefiting qi,expelling blood stasis and dissipating phlegm group weakened most obviously（P〈0.05）. Conclusion The therapeutics of benefiting qi,expelling blood stasis and dissipating phlegm can significantly inhibit bleomyein induced pulmonary fibrosis in rats,and the possible mechanism was by inhibiting TGF-βand PAI-1 expression in the fibrotic process.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第19期1903-1905,共3页
Chongqing medicine
基金
国家自然科学基金预研项目(NSFYY201108)
关键词
肺纤维化
转化生长因子Β1
纤溶酶原激活物抑制物1
益气化痰化瘀法
pulmonary fibrosis
transforming growth factor β1
plasminogen activator inhibitor lithe therapeutics of benefiting qi,expelling blood stasis and dissipating phlegm
