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环介导等温扩增技术在致病菌检测中的应用 被引量：2

Application of LAMP in detection of pathogenic microbe
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摘要 环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是近10年来发展起来的具有简便、快速、灵敏度高、特异性强等优点的新的恒温核酸扩增技术,已被应用于致病菌检测的研究。LAMP技术需针对靶基因的6个区域设计4种特异性引物,并采用链置换型DNA聚合酶,操作步骤少,不需要聚合酶链反应(polymerase chain reaction,PCR)技术复杂的设备。本文就LAMP技术在致病菌检测中的应用作一综述。 Loop-mediated isothermal amplification （LAMP） is a novel method of nucleic acid amplification developed in the last 10 years, with the advantages such as simplicity, rapidity, high sensitivity and high specificity. It is always applied in the detection of pathogenic microbe. This method employs a DNA polymerase with strand displacement activity and a set of 4 different primers specifically designed to recognize 6 distinct regions on the target DNA, with fewer steps and simpler equip- ment compared to PCR assays. This paper summarized the applications of LAMP method in the detection of pathogenic microbe.
作者 贺尔娜 蔡挺 张顺
机构地区 宁波大学医学院 宁波大学附属第二医院
出处 《中国人兽共患病学报》 CAS CSCD 北大核心 2012年第7期739-744,共6页 Chinese Journal of Zoonoses
关键词 环介导等温扩增(LAMP) 致病菌 检测 LAMP pathogenic microbe detection
分类号 R378 [医药卫生—病原生物学]
  • 相关文献

参考文献35

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二级参考文献36

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共引文献52

同被引文献42

  • 1韩宏蕾,施心路,白昕.微型生物在水质监测中的作用和意义[J].环境科学与管理,2007,32(5):147-150. 被引量：12
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引证文献2

二级引证文献2

中国人兽共患病学报
2012年 第7期

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