摘要
目的:了解杭州市萧山区不同来源金黄色葡萄球菌(Staphylococcus aureus,SA)分离株主要肠毒素SEA、SEB、SEC、SED基因携带情况。方法:应用环介导等温扩增(Loop-mediated isothermal amplification,LAMP)方法对34株分别从医院(物体表面)消毒监测样品、食品监测样本及食物中毒现症病人粪便和呕吐物中分离到的SA菌株进行肠毒素SEA、SEB、SEC、SED基因检测。结果:34株SA菌株中,肠毒素基因检出率为58.82%,其中SEA、SED检出率较高,分别为26.47%和32.35%。不同来源SA分离株肠毒素基因检出率有所不同,20株来自医院消毒监测样品SA中检出肠毒素基因12株,检出率为60.00%;9株来自食品监测样本SA中检出肠毒素基因3株,检出率为33.33%;5株来自食物中毒SA中检出肠毒素基因5株,检出率为100%。结论:34株实验分离株SA中肠毒素基因检出率较高,且可同时分泌两种毒素(10/34)。试验表明,LAMP技术应用于检测SA分离株中肠毒素基因简单、快速、易行,极适合在基层实验室应用。
Objective：To understand enterotoxigenic genes（SEA,SEB,SEC,SED） in Staphylococcus aureus（SA） isolates from different sources in Xiaoshan District of Hangzhou City.Methods：34 SA isolates were isolated from hospital disinfection monitoring samples,food monitoring samples and the vomit and faecal matter from food poisoning patients,and then the SE genes（SEA,SEB,SEC,SED） were detected by Loop-mediated isothermal amplification（LAMP）.Results：The detection rate of SE genes was 58.82%,and that of SEA and SED 26.47% and 32.35%,respectively.SE genes from different sources had different detection rates.SE genes were positive in 12 of 20 SA from hospital disinfection monitoring samples（60%）.SE genes were positive in 3 of 9 SA from food monitoring samples（33.33%）.SE genes were positive in 5 of 5 SA from food poisoning samples（100%）.Conclusion：The positive rate of SE genes is high in 34 strain SA,10 of 34 strains can be detected for two kinds of SE in the same strain.LAMP can be applied to detect SE in SA simply,rapidly and conveniently and used in grassroots laboratory.
出处
《中国卫生检验杂志》
CAS
2010年第5期1088-1089,1097,共3页
Chinese Journal of Health Laboratory Technology
关键词
金黄色葡萄球菌
肠毒素基因
环介导等温扩增技术
Staphyloccocus aureus
Enterotoxin gene
Loop-mediated isothermal amplification
