摘要
为研究甲砜霉素在鸡肌肉中的残留消除规律,鸡肌肉样品经丙酮、二氯甲烷提取,饱和正己烷脱脂,氮吹仪吹干浓缩后用流动相溶解,上高效液相色谱荧光检测器检测。测定鸡肌肉样品中甲砜霉素的检测限为1.5μg.kg-1(S/N=3)、定量限为5μg.kg-1(S/N=10)。各试验组鸡分别按体质量以10.0、20.0和50.0mg.(kg.d)-1剂量给药,每天1次,连续5d内服给药后,鸡肉中甲砜霉素残留量随给药剂量增大呈上升趋势,随休药期的延长而降低。休药第1天时,鸡肉中甲砜霉素残留量均达到峰值。休药前期甲砜霉素残留消除较快,后期消除缓慢。休药第5天时,鸡肉中甲砜霉素残留量均低于50μg.kg-1,休药第9天时,鸡肌肉中甲砜霉素残留量均低于检测限(1.5μg.kg-1);甲砜霉素在鸡肌肉中的残留量与给药剂量呈正相关。
A study on thiamphenicol（TAP） residue depletion was conducted in chicken muscle.TAP was extracted from chicken muscle with acetone and dichloromethane.The extract solution was degreased in n-hexane saturated with acetonitrile,dried in nitrogen evaporator and residues were dissolved in mobile phase.TAP was determinated by high performance liquid chromatography（HPLC） with fluorescence detector.The limits of detection（LODs） was 1.5 μg·kg-1（S/N=3） and the limits of quantitation（LOQs）was 5 μg·kg-1（S/N=10） for TAP in chicken muscle,respectively.After the chickens orally administered successively TAP capsules of 10.0,20.0 and 50.0 mg·kg-1 of body weight once every day for 5 days,respectively,residues of TAP in chicken muscle had a rise trend.The maximum residue of TAP was detected at the first day after withdrawal time in chicken muscle.At the early days after withdrawal time,the residues of TAP in chicken muscle were eliminated faster,but they were eliminated slowly at the later period.Residues of TAP in chicken muscle were all lower than 50 μg·kg-1 at the 5th withdrawal day,and all lower than the lowest LODs（1.5 μg·kg-1）at the 9th withdrawal day.Residues of TAP in chicken muscle were positively correlated with TAP orally administered doses.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第7期1150-1155,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省科技成果转化专项(BA2008071)
国家肉鸡产业技术体系专项(CARS-42-G23)
江苏高校优势学科建设工程资助项目
关键词
鸡肌肉
甲砜霉素
残留
消除
高效液相色谱荧光检测法
chicken muscle； thiamphenicol； residue； depletion； HPLC with fluorescence detection
