摘要
探讨桦褐孔菌多糖对感染弓形虫小鼠基因表达谱的影响,并对其抗虫机理进行分析。BABL/C小鼠腹腔接种RH株弓形虫速殖子103个,建立小鼠弓形虫感染模型。将小鼠随机分成3组:空白对照组、感染对照组、桦褐孔菌多糖治疗组。采集动物肝脏迅速冻存,提取总RNA,检测RNA质量,扩增RNA,进行芯片杂交,扫描后进行数据分析。结果:经SOM分析筛选出桦褐孔菌多糖治疗组的有效差异基因,并对其进行功能注释,分析其治疗弓形虫的主要原因。结果表明:桦褐孔菌多糖可有效治疗弓形虫感染引起的病理损伤,其抗弓形虫机理是通过调节宿主细胞因子水平,促进Th1/Th2平衡,控制Toll样受体信号通路以及对机体整体调控达到抗弓形虫目的。
The objective of this study was to observe the effects of polysaccharides in Inonotus obliquus on differential gene expression profiling in mouse infected with Toxoplasma gondii and discuss the mechanism of inhibiting Toxoplasma gondii.BABL/C mice were inoculated intraperitoneally with 103 tachyzoites of Toxoplasma gondii of RH strain to establish a mouse model of infection of toxoplasmosis.The mice were randomly divided into three groups： normal control group,negative control group,Inonotus obliquus polysaccharide group.Animal liver were collected and frozen-stored quickly.Its RNA was extracted and amplified,then hybridization was conducted.The figures were scanned for data analysis.The results were as follows： The effective differential genes of polysaccharide group were searched out by SOM analysis.The effective differential genes were annotated on gene functional aspect.The main reasons of which Inonotus obliquus polysaccharide inhibited Toxoplasma were announced.The mechanism that the effects of polysaccharides in Inonotus obliquus inhibited Toxoplasma gondii are achieved by effectively regulating the cytokine levels of mice,promoting Th1/Th2 balance,controlling Toll-like receptor signaling pathways and integral controlling the body.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第7期1134-1142,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30860211)
关键词
桦褐孔菌多糖
弓形虫
基因表达谱
抗虫机理
polysaccharides in Inonotus obliquus
Toxoplasma gondii
gene expression profiling
