摘要
为实现无籽西瓜品种的快速、准确鉴定,用23对西瓜核心SSR引物分析了15份无籽西瓜品种的DNA指纹。23对核心引物中有21对具多态性,共扩增出58个基因型,平均2.71个,平均多态性信息量(PIC)为0.36。10对引物在9个品种上具有特征谱带,组合PIC值>0.4的8对引物可以将参试的15个无籽西瓜品种一一区分开,并利用这8对引物构建了参试品种的数字指纹图谱。研究表明,西瓜核心SSR引物适合用于构建无籽西瓜品种的DNA指纹,将为无籽西瓜品种真实性鉴定和知识产权保护提供有效的技术依据。
Fifteen Zhengkang triploid seedless watermelon varieties were fingerprinted using the published 23 core SSR marker primer pairs for watermelon. Twenty one out of 23 primer pairs were polymorphic among tested triploids and produced 58 genotypes with an average of 2.71 per pair. The mean PIC was 0.36. Ten primer pairs produced unique bands for nine varieties. All 15 varieties were distinguished by 8 primer combinations,PIC 〉 0.4,and their digital fingerprinting code was also established. The results indicate that the published core SSR markers are suitable for the construction of DNA fingerprinting of triploid seedless watermelon varieties. The fingerprinting will provide reliable evidence for the variety identification and protection of intellectual property right for these varieties.
出处
《中国瓜菜》
CAS
2012年第4期1-4,共4页
China Cucurbits And Vegetables
基金
现代农业产业技术体系建设专项(CARS-26-03)
关键词
无籽西瓜
SSR
指纹图谱
Seedless watermelon
SSR
Fingerprinting
