摘要
用间接ELISA试剂盒对北京市郊区所采集的174份猪血清样品进行了抗体检测,结果显示,其抗体总阳性率达62.1%。针对戊型肝炎病毒(HEV)ORF2/ORF3重叠区设计了简并引物,采用巢式RT-PCR对随即采取的少量猪粪便样品进行了抗原检测。结果表明,部分粪便样品中存在HEV。
To investigate hepatitis E virus（HEV） infection in pigs in Beijing suburbs,we collected 174 swine sera,and tested antibodies using ELISA kit.The results demonstrate that the total positive rate of anti-HEV antibodies was 62.1%.Responses in a nested RT-PCR assay to a pair of degenerated primers designed from ORF2/ORF3 overlapping region indicated presence of gene sequence of HEV in the stool samples.
出处
《中国兽医杂志》
CAS
北大核心
2012年第6期6-9,共4页
Chinese Journal of Veterinary Medicine
基金
国家自然科学基金资助项目(30871582)
中国科技部(MOST)"国家国际科技合作计划"(2009DFA32710)
