摘要
目的了解广西地区猪、鼠和狗血清中抗-戊型肝炎病毒(HEV)IgG抗体流行情况。方法应用HEV基因1型开放读码框(ORF)2和ORF3多肽包被酶标板,辣根过氧化物酶(HRP)标记的羊抗猪、羊抗鼠和羊抗狗建立的酶联免疫吸附试验(ELISA)法检测猪、鼠和狗血清中抗-HEV IgG。结果总抗-HEVIgG阳性率为31.84%(170/534),其中猪血清抗-HEV IgG阳性率为26.40%(66/250),鼠血清抗-HEV IgG阳性率为43.02%(77/179),狗血清抗-HEV IgG阳性率为25.71%(27/105)。结论猪、鼠和狗血清中存在HEV感染,鼠抗-HEV IgG阳性率最高。
Objective To investigate hepatitis E virus （HEV） infection in swine, rats and dogs in Liuzhou of Guangxi. Methods Anti-HEV IgG was detected in sera of swine, rats and dogs by an enzyme-linked immunoabsorbent asaay （ELISA）, which was developed using the synthetic peptides derived from genotype 1 HEV ORF2 and ORF3 sequences. Results The total anti-HEV IgG positive rate was 31.84 % （170/534） from all animals tested. Anti-HEV IgG positive rates of swine, rats and dogs were 26.40% （66/250）,43.02% （77/179） and 25.71% （27/105） respectively. Conclusion Swine, rats and dogs could be infected by HEV, and the prevalence rate of anti-HEV IgG was the highest in rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第2期228-229,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30570063)
广西壮族自治区卫生厅立项课题(Z2005203)
