摘要
鹅出血性多瘤病毒(Goose hemorrhagic polyomavirus,GHPyV)是鹅出血性肾炎肠炎的致病病原,迄今为止,国际上已从鹅、番鸭和半番鸭中检测到GHPyV。为了解北京鸭是否存在GHPyV的感染,本研究从北京、山东、河北、河南等地的北京鸭种鸭场采集肝脾组织样品68份,并用GHPyV特异性PCR进行了检测。结果显示,从5份肝脾组织样品中检出GHPyV的VP1基因。选一株北京鸭源GHPyV(106株)测定了基因组序列。测序结果显示,北京鸭源GHPyV基因组长度为5 254bp,相对于德国鹅源GHPyV毒株Germany 2001,106株基因组在其编码区共有10个碱基位发生突变,但只有2个碱基位的突变引起氨基酸的置换;此外,106株基因组的非编码区存在2个核苷酸的缺失。对不同水禽来源的GHPyV基因组序列进行比较的结果表明,GHPyV的基因组序列具有高度保守性。由于GHPyV可感染鹅、番鸭、半番鸭和北京鸭,表明该病毒具有较广泛的宿主范围。
Goose hemorrhagic polyomavirus（GHPyV） is the causative agent of hemorrhagic nephritis and enteritis of geese（HNEG）.The virus has been detected in Muscovy and Mule ducklings.To investigate the prevalence of GHPyV in Pekin ducks in China,68 liver and spleen specimens were collected from Shandong,Beijing Hebei and Henan provinces and screened by GHPyV-PCR.Out of the 68 samples,5 were detected positive for GHPyV,demonstrating the presence of GHPyV in Pekin ducks in China.One positive sample was selected for whole genome characterization.Sequencing and sequence analysis indicated that the complete genome of strain 106 comprised 5254 bp.Compared with the sequence of GHPyV isolate Germany 2001,nucleotide substitutions were found at ten loci including two amino acid substitutions in the sequence of the Chinese isolate.In addition,two 1-bp deletions were detected in non-coding region of the Chinese isolate.The present study indicates the GHPyV has a broad host range and the GHPyV genome is relatively stable.Further studies are needed to determine the pathological significance of GHPyV infection in ducks.
出处
《中国兽医杂志》
CAS
北大核心
2012年第6期3-6,共4页
Chinese Journal of Veterinary Medicine
基金
现代农业产业技术体系专项资金(CARS-43)
公益性行业(农业)专项经费(201003012)
关键词
鸭
鹅出血性肾炎肠炎
鹅出血性多瘤病毒
Duck
Goose hemorrhagic polyomavirus
Hemorrhagic nephritis and enteritis of geese
