摘要
目的 对感染灶中的脆弱类杆菌进行快速检测。方法 首次使用抗脆弱类杆菌单克隆抗体池作为诊断试剂 ,采用免疫荧光法直接检测感染灶中的脆弱类杆菌。结果 对临床 82份感染标本的检测 ,用厌氧培养法检测阳性者 ,此法检测均为阳性 ,其一致性 85 .7%。厌氧培养法阳性率为 48.7% ,此法阳性率为5 7.3 % ,统计学处理二者差异显著 (P <0 .0 5 )。结论 此法特异性强 ,敏感性高 ,快速简便 ,避免了因细菌表面抗原变异而致单一单克隆抗体效价降低甚至“失效”的问题。
Objective To evaluate the method of rapid diagnosis of Bacteroides fragilis infection in clinical specimens.Methods Bacteroides fragilis infection of clinical speciments was examined with immunofluorescent assay using monoclonal antibody pool angainst B. fragilis as the diagnostic agent.Results Examination of 82 clinical specimens revealed that the positive rate for monoclonal antibody pool immunofluorescent assay was 57.3% and that for anaerobic culture was 48.7%(P<0.05). The consistent rate between the 2 methods was 85.7%.Conclusion Monoclonal antibody pool immunofluorescent assay is specific, sensitive and easy to perform. In addition, this method will not be affected by the decrease or loss of the efficiency of one kind of monoclonal antibodies and the survival conditions of B. fragilis cannot interfere with the normal operation of this method.
出处
《第三军医大学学报》
CSCD
北大核心
2000年第5期428-429,共2页
Journal of Third Military Medical University
基金
全军"九五"攻关课题资助项目!(96L042)
