摘要
目的 :观察疗前鼻咽癌组织中p5 3蛋白的积聚及其对瘤细胞有丝分裂和凋亡的影响。方法 :随机收集 1997年疗前鼻咽癌活检标本 43例。采用免疫组化LSAB法DO 7一抗检测 p5 3蛋白的表达。在H&E染色切片上计数有丝分裂图像 ,平均每个高倍视野下的瘤细胞有丝分裂数为有丝分裂指数 (mitoticindex ,MI)。采用TUNEL原位细胞死亡试剂盒检测瘤细胞凋亡 ,平均每个高倍视野下的凋亡瘤细胞数为凋亡指数 (TUNELindex ,TI)。比较高于和低于不同 p5 3阳性瘤细胞百分率的两组间MI均数有无差异 ,以确定 p5 3蛋白积聚的标准。比较有无p5 3蛋白积聚两组间的MI均数和TI均数。结果 :在 43例疗前鼻咽癌活检标本中 ,见到p5 3阳性瘤细胞≥2 0 %以上时 ,两组MI均数开始显示有差异 ,从而将 p5 3阳性瘤细胞≥ 2 0 %定为 p5 3蛋白积聚。 43例中有 37例p5 3蛋白积聚 ,积聚率为 86 0 5 % (37/4 3)。 37例有p5 3蛋白积聚组的MI均数为 1 87± 1 78/HPF ,6例无 p5 3蛋白积聚组MI均数为 0 76± 0 6 3/HPF ,两者有显著性差异 ,P <0 0 5。但是 ,37例TI的均数为 2 4 5 0± 2 6 6 6 /HPF ,6例TI的均数为 2 3 17± 2 5 30 /HPF ,统计学上无差异性。结论 :在疗前鼻咽癌活检中 ,p5 3蛋白阳性瘤细胞数≥ 2 0 %时可定为p5 3蛋白积聚 ,积聚率达
Objective:To investigate the p53 protein accumulation and its effects on neoplastic cell mitosis and apoptosis in pretreated nasopharyngeal carcinomas Methods:Forty three pretreated NPC biopsy samples in the year 1997 were randomly collected for this study p53 protein expression was detected by LSAB immunohistochemistry using DO 7 primary antibody Mitotic figures were counted on H&E stained slides, and apoptotic cells on TUNEL stained slides by use of in situ cell death detection kit Both of mitotic and apoptotic cells were quantitated by cell numbers per one high power field averagely in terms of mitotic index (MI) and TUNEL index (TI), respectively To compare the mean MIs of two groups categorized by different percentages of positive p53, positive cells found in NPC specimens was taken for the purpose of designating the criterion of p53 accumulation And then, the correlation of p53 accumulation with MI and TI was made by statistical analysis Results:Because significant difference appeared at the criterion of 20%, the p53 protein accumulation of NPC was defined as ≥20%of positive cells The p53 protein accumulation thus could be determined in 37 out of 43 NPCs(86 05%) The mean MI (1 87±1 78/HPF) of 37 NPCs with p53 accumulation was significantly higher than that (0 76±0 63/HPF) of 6 NPCs without p53 accumulation ( P< 0 05) However, there was no statistical difference between the mean TI (24 50±26 66/HPF) of 37 NPCs with p53 accumulation and TI (23 17±25 30/HPF) of 6 NPCs without p53 accumulation Conclusions:p53 protein accumulation of NPC could be designated by ≥20%of positive neoplastic cells found in pretreated NPC specimens The accumulated p53 could enhance cell proliferative activity in pretreated NPCs represented by increasing of MI, but showed no effect on neoplastic cell apoptosis
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第5期432-435,445,共5页
Chinese Journal of Cancer
基金
国家自然科学基金!(3930 2 0 0 -II)资助
