摘要
目的 了解国人结直肠癌及正常肠粘膜p16基因 5′CpG岛甲基化状况 ,并探讨其意义。方法 用甲基化敏感的限制性内切酶消化结合PCR法检测 6 0例结直肠癌及其正常结直肠粘膜p16基因 5′CpG岛中HpaⅡ、SacⅡ、SmaⅠ位点的甲基化状况。结果 结直肠癌与正常结直肠粘膜的p16基因 5′CpG岛均有不同程度的甲基化 ,比较结直肠癌与正常结直肠粘膜的p16基因甲基化状态及其与临床病理资料的关系 ,差异均无显著性。正常结直肠粘膜的p16基因甲基化状态在不同的酶切位点存在差异 (P <0 .0 5 )。结直肠粘膜甲基化与年龄有相关趋势。结论 结直肠粘膜的p16基因甲基化在不同的酶切位点间存在差异。正常结直肠粘膜和结直肠癌的p16基因甲基化状态是比较复杂的。
Objective To investigate the methylation status and its significance of 5′CpG island of p16 gene in colorectal carcinoma and normal colonic mucosa. Methods PCRbased methylation assay was applied to examine a total of 60 samples of colorectal carcinoma and paired 60 samples of the normal colonic mucosa for p16 gene methylation in Hpa Ⅱ, Sac Ⅱ, Sma Ⅰ sites. Results Methylation was identified in 5′CpG island of p16 gene in both colorectal carcinoma and normal colonic mucosa, but no significant difference obtained between normal mucosa and carcinoma. The methylation status in 5′ CpG island of p16 gene in colorectal carcinoma was not related to the tumor size, type, and metastases of the lymph nodes. Nevertheless, there was a significant difference between the methylation status of Hpa Ⅱ, Sac Ⅱ and Sma Ⅰ sites. Conclusions The methylation in 5′ CpG island of p16 gene in colonic mucosa is different in various restriction enzyme sites, and the methylation process of p16 gene in both normal colonic mucosa and colorectal carcinomas is complicated. Anyhow, there is a tendency that the increase of methylation in p16 gene is age dependent.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2000年第2期95-98,共4页
Chinese Journal of Pathology
基金
卫生部科研基金资助项目!(96-1-349)
杭州铁路分局科研基金资助项目
