摘要
目的 观察非小细胞肺癌组织中的自发性癌细胞凋亡及增殖细胞核抗原 (PCNA)、微血管密度对自发性癌细胞凋亡影响。方法 应用末端脱氧核糖核苷酰转移酶 (TDT)原位末端标记法显示凋亡细胞 ,免疫组化ABC法显示PCNA及微血管。结果 观察 46例非小细胞肺癌 ,自发性癌细胞凋亡发生率平均为 2 .8%± 2 .3% ,33例鳞癌自发性癌细胞凋亡 ( 3.4%± 1.7% )显著高于 13例腺癌( 1.2 %± 2 .0 % ) ,11例低分化癌自发性癌细胞凋亡 ( 3.9%± 2 .7% )显著高于 2 9例中分化癌 ( 2 .8%±2 .0 % )及 6例高分化癌 ( 0 .5 %± 0 .2 % ) ,2 7例PCNA阳性细胞百分率≥ 2 5 %的肺癌自发性癌细胞凋亡( 3.4%± 2 .2 % )显著高于 19例PCNA阳性细胞百分率 <2 5 %的肺癌 ( 1.9%± 2 .2 % ) ,2 6例自发性癌细胞凋亡发生率≥ 2 %的肺癌微血管密度 ( 42 .0± 12 .8/ 2 0 0倍视野 )显著低于 2 0例自发性癌细胞凋亡发生率 <2 %的肺癌 ( 74.4± 2 9.6 / 2 0 0倍视野 ) ,镜下癌细胞凋亡多见于微血管密度较低的区域及远离微血管的部位。结论 肺癌细胞自发性凋亡在鳞癌、低分化及增殖快的癌组织内发生率高 ,缺血。
Objective To observe the spontaneous apoptosis in human nonsmall cell lung cancer (NSCLC) tissues, and the impact of PCNA and microvessel density on it. Methods TDT in situ labeling and immunohistochemisty were used to display apoptotic cells, PCNA and microvessels. Results The total fortysix cases of NSCLC were observed. Apoptosis index (AI) was 2.8%±2.3% on average, and higher in squamous carcinomas (3.4%±1.7%) than in adenocarcinomas (1.2%±2.0%). AI in the poorly differentiated carcinomas (39%±2.7%) was significantly higher than in the middle and welldifferentiated carcinomas (2.8%±2.0%, 05%±0.2% respectively). AI was higher in NSCLC with PCNApositive cell rate ≥25% (3.4%±2.2%) than with PCANpositive cell rate <25% (1.9%±2.2%). Microvessel density was higher in NSCLC with AI<2% ( 74.4±29.6 counts/200 field) than with AI≥2% (42.0±12.8 counts/200 field). Apoptotic cells were frequently seen in the regions with less microvessels and distal to microvessels. Conclusions Spontaneous apoptotic cells mainly occur in the tissues of squamous carcinomas and poorlydifferentiated carcinomas and NSCLC with higher PCNApositive cell rates. Hypoxia could induce apoptosis of lung cancer cells.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2000年第2期92-94,共3页
Chinese Journal of Pathology
基金
国家自然科学基金资助项目!(39570788)
