摘要
利用20对SSR引物对来自辽宁省14个地级市的963份野生大豆种质进行了遗传多态性分析。结果共检测到等位基因变异141个,每对引物等位基因变异范围4～11个。等位基因的平均频率是0.1326,范围是0.0016～0.9045。各地区间遗传相似度变幅为0.6730～0.8589,其中沈阳和铁岭的遗传相似性最大;其次是大连和鞍山、辽阳和鞍山;丹东和朝阳、抚顺和大连的遗传相似度最低。聚类分析中,14个地区共分为2个大类和4个小类,表明材料的遗传背景与地理分布具有一定的相关性。
The genetic diversity of 963 wild soybean germplasms from 14 regions in Liaoning province was analyzed using 20 pairs of SSR primers.A total of 141 variation were detected in alleles with the frequency of 4-11 per pair of primer.The averaged frequency of allele was 0.1326 in the range of 0.0016-0.9045.The genetic similarity index of wild soybeans in different regions varied from 0.6730 to 0.8589,with that between Shenyang and Tieling the highest;followed by Dalian and Anshan,Liaoyang and Anshan;Dandong and Chaoyang,Fushun and Dalian was the smallest.The 14 areas were divided into 2 major categories and 4 sub-categories in clustering analysis,which showed that the genetic background of germplasm was correlated to its geographical distribution to some extent.
出处
《大豆科学》
CAS
CSCD
北大核心
2012年第3期368-373,共6页
Soybean Science
基金
公益性行业(农业)科研专项经费资助(201003021)
