摘要
研究了在金属离子存在下司帕沙星在胶束体系中的荧光特性，首次发现锌（Ⅱ）和阴离子表面活性刑十二烷基硫酸钠对司帕沙星的荧光有显著的协同增敏作用。据此建立了测定人体尿液中司帕沙星含量的同步-导数荧光光谱法，定量线性范围为0．04－4．0mg／L，检测限为0．04mg/L。相对标准偏差为1．6％－3．9％。探讨了锌（Ⅱ）和十二烷基硫酸钠对司帕沙星荧光的增敏机理。
The fluorescence characteristics of complexes of sparfloxacin(SPFX) with metal ions Zn(Ⅱ),Mg(Ⅱ), Cd(Ⅱ) etc in micellar systems have been studied in details. The experiments indicated that zinc ioncould form stable fluorescent complexes with sparfloxacin. The fluorescence intensity of Zn/SPFXcomplexes in sodium dedecyl sulfae (SDS) system was 28-fold higher than that of sparfloxacin itself. Anew method using the first order derivative synchronous fluorimetry has been developed for thedetermination of sparfloxacin in human urine. The linear range was 0.04 mg/L ~ 4. 0 mg/L and thedetection limit was 0. 004mg/L and the RSD was 1. 6% ~ 3. 9%. The meachanism for enhancing thefluorescence instensity of SPFX by both of zinc ion and SDS was discussed.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第4期403-406,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!9875016
山西省自然科学基金!981006
关键词
司帕沙星
锌
抗菌素药物
同步-导数荧光光度法
Sparfloxacin, zinc ions, sodium dodecyl sulfate, first order derivative synchronous fluorescence
