摘要
目的 :为探讨重庆地区原发性高血压与血管紧张素转换酶基因 (ACE)插入 /缺失 (I/D)多态性的关系。方法 :采用多聚酶链反应直接扩增ACE基因第 16内含子 ,对 114例正常人和 75例原发性高血压患者进行分析 ,得到三种基因型 :纯合插入型 (II型 )、纯合缺失型 (DD型 )、杂合型 (ID型 )。结果 :原发性高血压患者ACE基因的DD型频率明显高于正常人 (χ2=13.75 ,P <0 .0 0 5 ) ,D等位基因频率也明显高于正常人 (χ2 =14.3 ;P <0 .0 0 5 ) ,统计学分析差异有显著性意义。结论 :ACE基因的缺失型 (DD型 )
Objective :To study the association of the angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism with essential hypertension in Chongqing. Methods:We use polymerase chain reaction (PCR) method to amplify sixteenth intron of ACE gene to analyse 114 healthy controls and 75 patients with essential hypertension. Polymorphism was characterized by three genotypes: homozygous alleles DD, II, and heterozygous alleles ID. Results:The frequency of homozygous alleles DD and the frequency of deletion alleles were significantly higher in the essential hypertension group than in the healthy controls (P<0.005). Conclusion:The results indicate that a deletion polymorphism of ACE gene is associated with essential hypertension in Chongqing.
出处
《重庆医科大学学报》
CAS
CSCD
2000年第2期184-185,207,共3页
Journal of Chongqing Medical University
关键词
血管紧张素转换酶
基因多态性
原发性高血压
Angiotensin converting enzyme (ACE)
Gene polymorphism
Essential hypertension
