摘要
取矽肺(5例)、结节病(5例)及肺肿瘤(13例)病人的枝气管肺泡灌洗液中的巨噬细胞,在24孔培养板中培养24小时,测定培养上清液中细胞自发释放的或经LPS 刺激后释放的IL-1含量,同时也对这些病人外周血中的T 细胞及其亚类作了计数检查。结果表明,矽肺病人肺泡巨噬细胞自发的或经LPS 刺激后产生IL-1的量均高于非矽肺的两种疾病组病人的相应值,可能具有一定的鉴别诊断意义。这三组病人血中的T 细胞总数及T_4细胞数较正常减少,反映他们的T 细胞免疫功能受到一定程度的继发损害。
Macrophages obtained from bronchealalveolar lavage of patientswith silicosis (5cases).lung tumors(13cases) or sarcoidosis (5cases) werecultured with or without LPS for 24 hours.The supernatants from thecultures were titered for activity of IL-1.The results showed that bothspontaneous and LPS-stimulated release of IL-1 from lavage macrophages increased markedly in the patients with silicosis compared with theother groups of patients.Perhaps,this finding may have some signific-ance for differential diagnosis between pulmonary disordsrs.The numbersof T cells and T_4 cells of peripheral blood mononuclear cells weresignificantly decreased in all the three groups of patients compared withnormal values respectively.The relationship between the pathogenesis ofsilicosis and immunity of the patients needs more intensive study.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1990年第3期132-133,148,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金
关键词
矽肺
细胞
免疫
检测
silicosis
Aiveolar lavage macrophage
Interleukin 1
T cell and T cell subsets
