摘要
目的：测定猪血小板衍生生长因子的氨基酸组成和等电点。方法：用酸水解及聚丙烯酰胺凝胶等电聚焦的方法测定猪血小板衍生生长因子的氨基酸含量、组成成分和等电点。结果：酸水解法测定的猪血小板衍生生长因子含有１７种不同的氨基酸，其中含有相对较多的碱性氨基酸，其等电点为９．８～１０．３。结论：猪血小板衍生生长因子是一种强阳离子多肽，含有相对较多的碱性氨基酸，与其等电点为９．８～１０．３是一致的。
Objective: To determine the amino acid composition and isoelectric point of porcine platelet-derived growth factor. Methods: In the present experiment, acid dehydroxy amino acid analysis and isoelectric focusing in polyacylatmide gels were used and the amino acid composition and isoelectric point of porcine platelet-derived growth factor were studied. Results: The findings of acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels showed that, porcine platelet-derived growth factor contained 17 amino acid residues in which the protein was rich in the strong predominance of basic amino acid and its isoelectric point was in the range of 9. 8-10. 3. Conclusion: Porcine platelet-derived growth factor is a strongly positive ionic polypeptide and may contain the strong predominance of basic amino acids in its amino acids composition, as reflected by the isoelectric point range of 9. 8-10. 3.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第4期369-371,共3页
Academic Journal of Second Military Medical University
关键词
血小板衍生生长因子
氨基酸组成
等电点
platelet-derived growth factor
amino acid, sequencing isoelectric focusing
isoelectric point
