摘要
目的 观察丁咯地尔盐酸盐 (FZ)治疗新生鼠缺氧缺血性脑病 (HIE)的疗效 ,寻求治疗HIE的有效手段。方法 建立新生鼠HIE模型 ,将 10 4只新生鼠分为 13组 ,观察不同时间FZ 治疗后HIE鼠脑灌流状态、脑花生四烯酸、自由基清除、细胞内游离钙代谢 ,以及脑组织病理变化。结果 治疗组用FZ 前后颈动脉血压无变化 ,缺血侧半球软脑膜血管扩张占 18.6 %～ 2 2 .7% ,较对侧正常半球软脑膜血管扩张程度大 2倍 ,脑组织TXB2 /PGI2 比值、胞内游离钙含量减少 ,超氧化物歧化酶 (SOD)活性增加。电镜下神经细胞基本恢复正常。结论 FZ 可有效恢复缺血区脑灌流 ,而不发生脑内窃血现象 ,同时还可通过提高SOD活性及钙离子弱拮抗作用 ,减轻或避免再灌注损伤。
Objective To observe the effects of fonzylane on newborn rats with hypoxic ischemic encephalopathy (HIE) and search for an effective therapy to deal with HIE. Methods Models of newborn rats with HIE were established, and 104 rats were devided into 13 groups. After treatment with fonzylane,the blood perfusion, pathology, arachidonic acid content, free radical clearance, and metabolism of intracellular free calcium in the brain of rats were investigated. Results Carotid arterial blood pressures of rats were not different before and after applying fonzylane. The arteriolae of the soft meninges of ischemic cerebral hemisphere were dilated by 18.6 %～22.7 %,which were two times more than those of the other normal cerebral hemisphere; the blood circulation was also significantly accelerated; both the ratio of TXB 2/PGI 2 and the content of the endocellular free calcium decreased; but the SOD activity of cerebrum increased. The neurocytes were observed almost recovered under the electronmicroscope. Conclusion The blood perfusion of the ischemic region of cerebrum could be significantly recovered by using fonzylane. There was no intracerebral steal phenomenon. In the mean time fonzylane, as a mild calcium antagonist and activitor of SOD, could reduce or might avoid the reperfusion damage. The results indicated that further clinical investigations on the treatment of HIE neonates with Fonaylane are needed.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2000年第5期299-301,共3页
Chinese Journal of Pediatrics
