摘要
目的:研究母亲过敏性哮喘病史对新生儿调节性T细胞的影响。方法:收集62例胎儿脐带血[40例健康母亲(对照组),22例母亲有过敏性哮喘病史(哮喘组)],分离单个核细胞进行体外培养,每例样品均分别给予以下4种刺激:类脂A(LpA)-Toll样受体4的配体,肽多糖(Ppg)-Toll样受体2的配体,植物血凝素(PHA),屋尘螨提取物。培养3天后,应用流式细胞技术检测CD4+CD25+Foxp3+调节性T细胞数量;Luminex流式荧光仪检测特异性细胞因子分泌浓度;体外分离CD4+CD25+调节性T细胞与CD4+CD25-效应T细胞共同培养,检测调节性T细胞对效应细胞的增殖和分泌细胞因子的抑制功能。结果:在Ppg诱导的免疫反应中,与对照组相比,过敏组脐带血中调节性T细胞数量(CD4+CD25+Foxp3+T细胞)减少;相关细胞因子IL-10分泌降低,差异具有统计学意义。在PHA诱导的免疫反应中,与对照组相比,调节性T细胞对效应细胞增值的抑制功能下降,差异具有统计学意义;对于效应细胞分泌IL-13的抑制功能也趋向于降低。结论:母亲有过敏性哮喘病史的新生儿脐带血中,调节性T细胞的数量和功能存在缺陷,可能与其易患过敏性疾病有关。
Objective：Examined the impairment of regulatory T cells in cord blood from offspring of allergic asthmatic mothers. Methods：Cord blood mononuclear cells from 62 healthy neonates（40 healthy mothers and 22 allergic asthmatic mothers） were isolated,and cultured with stimuli： lipid A（TLR4 ligands）,peptidoglycan（Ppg-TLR2 ligands）,mitogen（PHA,phytohemagglutinin） and Dermatophagoides pteronyssinus1.And then the amount of CD4＋CD25＋Foxp3＋T cells was acounted with flow cytometry;cytokine concentrations were measured in supernatants by LUMINEX technology;the suppressive function of regulatory T cells were examinated by isolating and culturing of CD4＋CD25＋T cells and CD4＋CD25-T cells in vitro. Results：Cord blood from offspring of allergic asthmatic mothers showed Ppg-induced fewer regulatory T cells（CD4＋CD25＋Foxp3＋T,P=0.03） and lower IL-10 secretion（P=0.03）.Furthermore,the suppressive capacity of regulatory T cells was impaired in PHA-induced division and proliferation of T effector cells in cord blood of offspring from allergic asthmatic mothers（P=0.05）.Meanwhile,the suppressive capacity of regulatory T cells to IL-13 production by effector cells was partially impared（P=0.07）. Conclusion：In offspring of allergic asthmatic mothers,regulatory T cells amount,and suppressive function were impaired at birth,which maybe potentially contribute to the the susceptibility to allergic diseases.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第6期560-563,共4页
Chinese Journal of Immunology
